Comparative Analysis of Pentacyclic Triterpenic Acid Compositions in Oleogum Resins of Different Boswellia Species and Their In Vitro Cytotoxicity against Treatment-Resistant Human Breast Cancer Cells

Pentacyclic triterpenic acids from oleogum resins of species are of considerable therapeutic interest. Yet, their pharmaceutical development is hampered by uncertainties regarding botanical identification and the complexity of triterpenic acid mixtures. Here, a highly sensitive, selective, and accur...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-06, Vol.24 (11), p.2153
Main Authors: Schmiech, Michael, Lang, Sophia J, Werner, Katharina, Rashan, Luay J, Syrovets, Tatiana, Simmet, Thomas
Format: Article
Language:English
Subjects:
Acids
Arthritis
Boswellia
Boswellia occulta
Boswellia sacra
boswellic acid
Breast cancer
Cluster analysis
Comparative analysis
Composition
Cytotoxicity
frankincense
Gene expression
High performance liquid chromatography
Kinases
Liquid chromatography
lupeolic acid
Mass spectrometry
Mass spectroscopy
MDA-MB-231
Metastases
Multivariate statistical analysis
pentacyclic triterpenic acid
Principal components analysis
Resins
Statistical analysis
Statistical methods
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Summary:Pentacyclic triterpenic acids from oleogum resins of species are of considerable therapeutic interest. Yet, their pharmaceutical development is hampered by uncertainties regarding botanical identification and the complexity of triterpenic acid mixtures. Here, a highly sensitive, selective, and accurate method for the simultaneous quantification of eight boswellic and lupeolic acids by high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) was developed. The method was applied to the comparative analysis of 41 oleogum resins of the species , , , , , , , , and . Multivariate statistical analysis of the data revealed differences in the triterpenic acid composition that could be assigned to distinct species and to their geographic growth location. Extracts of the oleogum resins exhibited cytotoxicity against the human, treatment-resistant, metastatic breast cancer cell line MDA-MB-231. Extracts from were the most potent ones with an average IC of 8.3 ± 0.6 µg/mL. The oleogum resin of the was further fractionated to enrich different groups of substances. The cytotoxic efficacy against the cancer cells correlates positively with the contents of pentacyclic triterpenic acids in extracts.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24112153