Therapeutic Use of Scoparia dulcis Reduces the Progression of Experimental Osteoarthritis

• Published in: Molecules (Basel, Switzerland) Switzerland), 2019-09, Vol.24 (19), p.3474
• Main Authors: Lima, Marcus Vinícius Viégas, Freire, Abner de Oliveira, Sousa, Emerson Lucas Frazão, Vale, André Alvares Marques, Lopes, Alberto Jorge Oliveira, Vasconcelos, Cleydlenne Costa, Lima-Aragão, Mônica Virginia Viégas, Serra, Humberto Oliveira, Liberio, Rosane Nassar Meireles Guerra, Santos, Ana Paula Silva de Azevedo Dos, Silva, Gyl Eanes Barros, Rocha, Claúdia Quintino da, Moreira Lima, Fernando César Vilhena, Cartágenes, Maria do Socorro de Sousa, Garcia, João Batista Santos
• Format: Article
• Language: English
• Subjects: Acids; Analgesics; Animal models; Animals; Arthritis; Biomarkers; Biomedical materials; Cartilage; Cartilage (articular); Cartilage diseases; Cartilage, Articular - drug effects; Cartilage, Articular - pathology; Chromatography, High Pressure Liquid; Cyclooxygenase-2; Cytokines; Cytokines - metabolism; Disease; Edema; Evaluation; Flavonoids; Flowers & plants; Health services; Histology; Inflammation; Knee; Meloxicam; Metabolites; Mice; Molecular docking; Osteoarthritis; Osteoarthritis, Knee - drug therapy; Osteoarthritis, Knee - etiology; Osteoarthritis, Knee - metabolism; Osteoarthritis, Knee - pathology; Pain; Pain perception; Plant Extracts - chemistry; Plant Extracts - pharmacology; Prostaglandin-Endoperoxide Synthases - genetics; Prostaglandin-Endoperoxide Synthases - metabolism; Rats; Scoparia - chemistry; Scoparia dulcis; Spectrometry, Mass, Electrospray Ionization; Synovial fluid; treatment; Tumor necrosis factor-TNF
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24193474

Pain is recognized as one of the main symptoms in knee osteoarthritis and is the main reason why patients seek medical attention. has been popularly used to relieve discomfort caused by various painful conditions. The objective of the study is to evaluate the analgesic and anti-inflammatory effect of the crude extract of , in an experimental model of osteoarthritis. The experiment was performed with Wistar rats divided into 4 groups with 5 animals each: healthy, saline, crude extract, and meloxicam groups. Knee osteoarthritis was induced by intra-articular injection of sodium mono-iodoacetate. First, clinical parameters of pain were assessed at days 0, 5, 10, 15, and 20 after induction. Second, the potential cyclooxygenase inhibition was evaluated, and the cytokines of the synovial fluid were quantified. An in silico test and Molecular Docking tests were performed. A histopathological evaluation was made on articular cartilage with safranin O staining. The results showed that a 15-day treatment with crude extract reduced edema, spontaneous pain, peripheral nociceptive activity, and proinflammatory cytokines in the synovial fluid. The highest inhibition of cyclooxygenase 2 in the crude extract occurred at 50 µg/mL. The crude extract of presents therapeutic potential for the treatment of osteoarthritis due to its anti-inflammatory and anti-nociceptive action.