Decreased Photosynthetic Efficiency in Nicotiana tabacum L. under Transient Heat Stress

• Published in: Plants (Basel) 2024-01, Vol.13 (3), p.395
• Main Authors: Falcioni, Renan, Chicati, Marcelo Luiz, de Oliveira, Roney Berti, Antunes, Werner Camargos, Hasanuzzaman, Mirza, Demattê, José A M, Nanni, Marcos Rafael
• Format: Article
• Language: English
• Subjects: Acclimatization; Acclimatization (Plants); Carbon dioxide; Carbon fixation; Chlorophyll; chlorophyll fluorescence; Comparative analysis; Crop resilience; Damage; dark respiration; Efficiency; Electron transport; electron transport chain; Energy dissipation; Environmental aspects; Environmental stress; Fluorescence; Gas analyzers; gas exchange; Heat; Heat shock proteins; Heat stress; Heat tolerance; Heat treatment; Heat treatments; Heating; High temperature; Infrared analysis; Leaves; Measurement; nonphotochemical quenching; Parameters; Photochemicals; Photosynthesis; Photosystem; Photosystem II; Physiological aspects; Physiology; Plant growth; Plant physiology; plant stress; Principal components analysis; Respiration; Temperature; Temperature effects; Thylakoids; Tobacco; Tobacco (Plant); Transport rate
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13030395

Heat stress is an abiotic factor that affects the photosynthetic parameters of plants. In this study, we examined the photosynthetic mechanisms underlying the rapid response of tobacco plants to heat stress in a controlled environment. To evaluate transient heat stress conditions, changes in photochemical, carboxylative, and fluorescence efficiencies were measured using an infrared gas analyser (IRGA Licor 6800) coupled with chlorophyll a fluorescence measurements. Our findings indicated that significant disruptions in the photosynthetic machinery occurred at 45 °C for 6 h following transient heat treatment, as explained by 76.2% in the principal component analysis. The photosynthetic mechanism analysis revealed that the dark respiration rate (Rd and Rd ) increased, indicating a reduced potential for carbon fixation during plant growth and development. When the light compensation point (LCP) increased as the light saturation point (LSP) decreased, this indicated potential damage to the photosystem membrane of the thylakoids. Other photosynthetic parameters, such as , , , and ΦCO , also decreased, compromising both photochemical and carboxylative efficiencies in the Calvin-Benson cycle. The energy dissipation mechanism, as indicated by the NPQ, qN, and thermal values, suggested that a photoprotective strategy may have been employed. However, the observed transitory damage was a result of disruption of the electron transport rate (ETR) between the PSII and PSI photosystems, which was initially caused by high temperatures. Our study highlights the impact of rapid temperature changes on plant physiology and the potential acclimatisation mechanisms under rapid heat stress. Future research should focus on exploring the adaptive mechanisms involved in distinguishing mutants to improve crop resilience against environmental stressors.