Phytochemical Screening Using LC-MS to Study Antioxidant and Toxicity Potential of Methanolic Extracts of Atraphaxis pyrifolia Bunge

• Published in: Molecules (Basel, Switzerland) Switzerland), 2024-09, Vol.29 (18), p.4478
• Main Authors: Abilkassymova, Alima, Aldana-Mejía, Jennyfer A, Katragunta, Kumar, Kozykeyeva, Raushan, Omarbekova, Ardak, Avula, Bharathi, Turgumbayeva, Aknur, Datkhayev, Ubaidilla M, Khan, Ikhlas A, Ross, Samir A
• Format: Article
• Language: English
• Subjects: Acids; Animals; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Arid regions; Artemia - drug effects; Atraphaxis species; Catecholamines; Chromatography; Chromatography, Liquid - methods; Cocoa; Cytotoxicity; Flavonoids; Flavonoids - analysis; Flavonoids - chemistry; glycosylated flavonoids; Health aspects; Identification; Instrument industry; Leaves; Liquid Chromatography-Mass Spectrometry; Mass spectrometry; Mass Spectrometry - methods; Medicine, Botanic; Medicine, Herbal; Metabolites; Methanol - chemistry; Phytochemicals; Phytochemicals - analysis; Phytochemicals - chemistry; Phytochemicals - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - toxicity; Plant Leaves - chemistry; Plant Stems - chemistry; Retention; Scientific imaging; therapeutic applications; Toxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29184478

, a native medicinal plant of Central Asia, has a long history of traditional medicinal use; however, scientific research on its phytochemical and biological properties remains scarce. This paper aims to elucidate its chemical profile and assess its pharmacological potential through a comprehensive investigation of the phytochemical composition of stems and leaves using Liquid Chromatography-Mass Spectrometry (LC-MS), in conjunction with the assessment of its antioxidant (DPPH and ABTS) and cytotoxicity test on . Predominantly, glycosylated flavonoids were detected in stems and leaves extracts, notably including 8-Acetoxy-3',4',5,5'-tetrahydroxy-7-methoxy-3-α-L-rhamno-pyranosyloxyflavone, pyrifolin, and dehydroxypyrifolin. While the latter compound is exclusive to , the former compounds serve as shared chemical markers with other species. The methanolic extracts of leaves exhibited significant antioxidant capacity without toxicity against . This study contributes to current research through providing valuable insights into the chemical diversity and potential medicinal properties of this plant species.