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Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis
Benzimidazole-based compound 2-( -tolyl)-1 -benzo[d]imidazole ( ) and its derivative probe have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of and compound were evaluated by UV-vis absorption and fluorescence spectroscopy...
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| Published in: | Biosensors (Basel) 2022-04, Vol.12 (4), p.224 |
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| Main Authors: | , , , |
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| creator | Song, In-Ho Yeom, Gyu Seong Kuwar, Anil Nimse, Satish Balasaheb |
| description | Benzimidazole-based compound 2-(
-tolyl)-1
-benzo[d]imidazole (
) and its derivative probe
have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of
and compound
were evaluated by UV-vis absorption and fluorescence spectroscopy.
showed high selectivity and sensitivity for Cys among tested analytes, including amino acids, anions, and cations.
selectively reacts with Cys and results in compound
with fluorescence turn-on effect.
did not show any interference from the components in the serum matrix for Cys detection in the human serum sample.
detects Cys in serum samples with 2.3-5.4-fold better LOD than reported methods. The detection limit of 86 nM and 43 nM in HEPES buffer using UV-visible and fluorescence spectroscopy, respectively, makes
an excellent chemosensor for Cys detection. |
| doi_str_mv | 10.3390/bios12040224 |
| format | article |
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-tolyl)-1
-benzo[d]imidazole (
) and its derivative probe
have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of
and compound
were evaluated by UV-vis absorption and fluorescence spectroscopy.
showed high selectivity and sensitivity for Cys among tested analytes, including amino acids, anions, and cations.
selectively reacts with Cys and results in compound
with fluorescence turn-on effect.
did not show any interference from the components in the serum matrix for Cys detection in the human serum sample.
detects Cys in serum samples with 2.3-5.4-fold better LOD than reported methods. The detection limit of 86 nM and 43 nM in HEPES buffer using UV-visible and fluorescence spectroscopy, respectively, makes
an excellent chemosensor for Cys detection.</description><identifier>ISSN: 2079-6374</identifier><identifier>EISSN: 2079-6374</identifier><identifier>DOI: 10.3390/bios12040224</identifier><identifier>PMID: 35448284</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino acids ; Anions ; Benzimidazoles ; biothiols ; Cations ; Chemical sensors ; Chemoreceptors ; Chromatography ; cysteine ; Cysteine - analysis ; Fluorescence ; Fluorescence spectroscopy ; Fluorescent Dyes - chemistry ; Homocysteine ; Humans ; Imidazole ; leukemia ; Limit of Detection ; Michael addition ; Selectivity ; serum ; Spectrometry, Fluorescence ; Spectroscopy ; Spectrum analysis</subject><ispartof>Biosensors (Basel), 2022-04, Vol.12 (4), p.224</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3934-a2b193d33c6cabd0b55ba7ea6b0c7dc88f803cc719f2be2ef6eb0ce0683e593</citedby><cites>FETCH-LOGICAL-c3934-a2b193d33c6cabd0b55ba7ea6b0c7dc88f803cc719f2be2ef6eb0ce0683e593</cites><orcidid>0000-0002-5137-0584</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2652956119/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2652956119?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53770,53772,74873</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35448284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, In-Ho</creatorcontrib><creatorcontrib>Yeom, Gyu Seong</creatorcontrib><creatorcontrib>Kuwar, Anil</creatorcontrib><creatorcontrib>Nimse, Satish Balasaheb</creatorcontrib><title>Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis</title><title>Biosensors (Basel)</title><addtitle>Biosensors (Basel)</addtitle><description>Benzimidazole-based compound 2-(
-tolyl)-1
-benzo[d]imidazole (
) and its derivative probe
have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of
and compound
were evaluated by UV-vis absorption and fluorescence spectroscopy.
showed high selectivity and sensitivity for Cys among tested analytes, including amino acids, anions, and cations.
selectively reacts with Cys and results in compound
with fluorescence turn-on effect.
did not show any interference from the components in the serum matrix for Cys detection in the human serum sample.
detects Cys in serum samples with 2.3-5.4-fold better LOD than reported methods. The detection limit of 86 nM and 43 nM in HEPES buffer using UV-visible and fluorescence spectroscopy, respectively, makes
an excellent chemosensor for Cys detection.</description><subject>Amino acids</subject><subject>Anions</subject><subject>Benzimidazoles</subject><subject>biothiols</subject><subject>Cations</subject><subject>Chemical sensors</subject><subject>Chemoreceptors</subject><subject>Chromatography</subject><subject>cysteine</subject><subject>Cysteine - analysis</subject><subject>Fluorescence</subject><subject>Fluorescence spectroscopy</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Homocysteine</subject><subject>Humans</subject><subject>Imidazole</subject><subject>leukemia</subject><subject>Limit of Detection</subject><subject>Michael addition</subject><subject>Selectivity</subject><subject>serum</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectroscopy</subject><subject>Spectrum analysis</subject><issn>2079-6374</issn><issn>2079-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdks1rFDEYhwdRbKm9eZaAFw-O5nOSXITtWnWhoLjeQz7eqVlmJmsyU9j-9c7u1rI1l7zk9-Qhb3ir6jXBHxjT-KOLqRCKOaaUP6vOKZa6bpjkz0_qs-qylA2el-RSM_myOmOCc0UVP6_ydRf7ONgxpgH9BOv3RX1lCwR0BcP9HAZ7nzpAP3JygNqU0XJXRogDoM8wwuECskNAq7GgxXbbRX-0xQGtIU89Wtt-OwsWg-12JZZX1YvWdgUuH_aLav3l-tfyW33z_etqubipPdOM15Y6ollgzDfeuoCdEM5KsI3DXgavVKsw814S3VIHFNoG5gRwoxgIzS6q1dEakt2YbY69zTuTbDSHg5Rvjc1j9B0YwUmQAVuhOObMS4eFA6-Jck4JKfHs-nR0bSfXQ_AwjNl2T6RPkyH-NrfpzmjMiKRiFrx7EOT0Z4Iymj4WD11nB0hTMbQRnCoppZrRt_-hmzTl-esOFNWiIWTf3fsj5XMqJUP7-BiCzX4yzOlkzPib0wYe4X9zwP4CnPm1zg</recordid><startdate>20220408</startdate><enddate>20220408</enddate><creator>Song, In-Ho</creator><creator>Yeom, Gyu Seong</creator><creator>Kuwar, Anil</creator><creator>Nimse, Satish Balasaheb</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5137-0584</orcidid></search><sort><creationdate>20220408</creationdate><title>Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis</title><author>Song, In-Ho ; Yeom, Gyu Seong ; Kuwar, Anil ; Nimse, Satish Balasaheb</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3934-a2b193d33c6cabd0b55ba7ea6b0c7dc88f803cc719f2be2ef6eb0ce0683e593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amino acids</topic><topic>Anions</topic><topic>Benzimidazoles</topic><topic>biothiols</topic><topic>Cations</topic><topic>Chemical sensors</topic><topic>Chemoreceptors</topic><topic>Chromatography</topic><topic>cysteine</topic><topic>Cysteine - analysis</topic><topic>Fluorescence</topic><topic>Fluorescence spectroscopy</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Homocysteine</topic><topic>Humans</topic><topic>Imidazole</topic><topic>leukemia</topic><topic>Limit of Detection</topic><topic>Michael addition</topic><topic>Selectivity</topic><topic>serum</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectroscopy</topic><topic>Spectrum analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, In-Ho</creatorcontrib><creatorcontrib>Yeom, Gyu Seong</creatorcontrib><creatorcontrib>Kuwar, Anil</creatorcontrib><creatorcontrib>Nimse, Satish Balasaheb</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biosensors (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, In-Ho</au><au>Yeom, Gyu Seong</au><au>Kuwar, Anil</au><au>Nimse, Satish Balasaheb</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis</atitle><jtitle>Biosensors (Basel)</jtitle><addtitle>Biosensors (Basel)</addtitle><date>2022-04-08</date><risdate>2022</risdate><volume>12</volume><issue>4</issue><spage>224</spage><pages>224-</pages><issn>2079-6374</issn><eissn>2079-6374</eissn><abstract>Benzimidazole-based compound 2-(
-tolyl)-1
-benzo[d]imidazole (
) and its derivative probe
have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of
and compound
were evaluated by UV-vis absorption and fluorescence spectroscopy.
showed high selectivity and sensitivity for Cys among tested analytes, including amino acids, anions, and cations.
selectively reacts with Cys and results in compound
with fluorescence turn-on effect.
did not show any interference from the components in the serum matrix for Cys detection in the human serum sample.
detects Cys in serum samples with 2.3-5.4-fold better LOD than reported methods. The detection limit of 86 nM and 43 nM in HEPES buffer using UV-visible and fluorescence spectroscopy, respectively, makes
an excellent chemosensor for Cys detection.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35448284</pmid><doi>10.3390/bios12040224</doi><orcidid>https://orcid.org/0000-0002-5137-0584</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Biosensors (Basel), 2022-04, Vol.12 (4), p.224 |
| issn | 2079-6374 2079-6374 |
| language | eng |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | Amino acids Anions Benzimidazoles biothiols Cations Chemical sensors Chemoreceptors Chromatography cysteine Cysteine - analysis Fluorescence Fluorescence spectroscopy Fluorescent Dyes - chemistry Homocysteine Humans Imidazole leukemia Limit of Detection Michael addition Selectivity serum Spectrometry, Fluorescence Spectroscopy Spectrum analysis |
| title | Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis |
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