Initial damage produced by a single 15-Gy x-ray irradiation to the rat calvaria skin

• Published in: European radiology experimental 2020-06, Vol.4 (1), p.32-10, Article 32
• Main Authors: da Silva Santin, Matheus, Koehler, José, Rocha, Danilo Massuia, dos Reis, Camila Audrey, Omar, Nadia Fayez, Fidler, Yasmin, de Miranda Soares, Maria Albertina, Gomes, José Rosa
• Format: Article
• Language: English
• Subjects: Animals; Cell growth; Cell Proliferation; Collagen; Diagnostic Radiology; Food irradiation; Imaging; Internal Medicine; Interventional Radiology; Male; Medicine; Medicine & Public Health; Morphology; Neuroradiology; Original; Original Article; Radiation Injuries - pathology; Radiobiology; Radiology; Rats; Rats, Wistar; Skin; Skin - radiation effects; Skull - radiation effects; Staining and Labeling; Ultrasound; X-Rays
• ISSN: 2509-9280; 2509-9280
• DOI: 10.1186/s41747-020-00155-4

Background Calvaria skin has a reduced thickness, and its initial damage produced by irradiation was scarcely reported. We aimed to identify the initial effects of x-ray irradiation in the rat calvaria skin. Methods After approval by the Animal Ethical Committee, calvaria skin sections of five Wistar rats per time point were evaluated on days 4, 9, 14, and 25 following a single 15-Gy x-ray irradiation of the head. The control group was composed of five rats and evaluated on day 4. Sections were assessed using hematoxylin-eosin and Masson’s trichrome staining for morphology, inflammation, and fibrosis. Fibrosis was also evaluated by the collagen maturation index from Picrosirius red staining and by cell proliferation using the immunohistochemistry, after 5-bromo-2-deoxyuridine intraperitoneal injection. Results In irradiated rats, we observed a reduction in epithelial cell proliferation ( p = 0.004) and in matrix metalloproteinase-9 expression ( p < 0.001), an increase in the maturation index, and with a predominance in the type I collagen fibers, on days 9 and 14 (1.19 and 1.17, respectively). A progressive disorganization in the morphology of the collagen fibers at all time points and changes in morphology of the sebaceous gland cells and hair follicle were present until day 14. Conclusions The initial damage produced by a single 15-Gy x-ray irradiation to the rat calvaria skin was a change in the normal morphology of collagen fibers to an amorphous aspect, a temporary absence of the sebaceous gland and hair follicles, and without a visible inflammatory process, cell proliferation, or fibrosis process in the dermis.