In silico analysis of virulence associated genes in genomes of Escherichia coli strains causing colibacillosis in poultry

Colibacillosis – the most common disease of poultry, is caused mainly by avian pathogenic (APEC). However, thus far, no pattern to the molecular basis of the pathogenicity of these bacteria has been established beyond dispute. In this study, genomes of APEC were investigated to ascribe importance an...

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Bibliographic Details
Published in:Journal of veterinary research 2017-12, Vol.61 (4), p.421-426
Main Authors: Kołsut, Joanna, Borówka, Paulina, Marciniak, Błażej, Wójcik, Ewelina, Wojtasik, Arkadiusz, Strapagiel, Dominik, Dastych, Jarosław
Format: Article
Language:English
Subjects:
amino acid sequences
Amino acids
Bacteria
birds
Colibacillosis
DNA
E coli
Escherichia coli
genes
Genomes
Nucleotide sequence
Pathogenicity
Phylogeny
Poultry
Strains (organisms)
veterinary medicine
Virulence
Virulence factors
virulence genes
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Summary:Colibacillosis – the most common disease of poultry, is caused mainly by avian pathogenic (APEC). However, thus far, no pattern to the molecular basis of the pathogenicity of these bacteria has been established beyond dispute. In this study, genomes of APEC were investigated to ascribe importance and explore the distribution of 16 genes recognised as their virulence factors. A total of 14 pathogenic for poultry strains were isolated, and their DNA was sequenced, assembled , and annotated. Amino acid sequences from these bacteria and an additional 16 freely available APEC amino acid sequences were analysed with the DIFFIND tool to define their virulence factors. The DIFFIND tool enabled quick, reliable, and convenient assessment of the differences between compared amino acid sequences from bacterial genomes. The presence of 16 protein sequences indicated as pathogenicity factors in poultry resulted in the generation of a heatmap which categorises genomes in terms of the existence and similarity of the analysed protein sequences. The proposed method of detection of virulence factors using the capabilities of the DIFFIND tool may be useful in the analysis of similarities of and other sequences deriving from bacteria. Phylogenetic analysis resulted in reliable segregation of 30 APEC strains into five main clusters containing various virulence associated genes (VAGs).
ISSN:2450-7393
2450-8608
2450-8608
DOI:10.1515/jvetres-2017-0051