Sustainable-Green Synthesis of Silver Nanoparticles Using Aqueous Hyssopus officinalis and Calendula officinalis Extracts and Their Antioxidant and Antibacterial Activities

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-11, Vol.27 (22), p.7700
• Main Authors: Balciunaitiene, Aiste, Puzeryte, Viktorija, Radenkovs, Vitalijs, Krasnova, Inta, Memvanga, Patrick B, Viskelis, Pranas, Streimikyte, Paulina, Viskelis, Jonas
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Antibacterial activity; Antibiotics; Antimicrobial activity; Antimicrobial agents; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Bacteria; Biosynthesis; Calendula; Calendula officinalis; Cancer; Drug resistance; Free radicals; Gram-negative bacteria; green synthesis; Hyssopus; Hyssopus officinalis; Hyssopus Plant; In vitro methods and tests; Metabolites; Metal Nanoparticles - chemistry; Microorganisms; Multidrug resistance; Nanoparticles; Pathogens; phytochemical analysis; Plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyethylene glycol; Scanning electron microscopy; Silver; Silver - chemistry; silver nanoparticles; Size distribution; Spectroscopy; Toxicity; Transmission electron microscopy
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27227700

Silver nanoparticles (AgNPs) biosynthesized using aqueous medical plant extracts as reducing and capping agents show multiple applicability for bacterial problems. The aim of this study was to expand the boundaries on AgNPs using a novel, low-toxicity, and cost-effective alternative and green approach to the biosynthesis of metallic NPs using and aqueous extracts. The formation of AgNPs was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) techniques. The effectiveness of biosynthesized AgNPs in quenching free radicals and inhibiting the growth of Gram-positive and Gram-negative microorganisms was supported by in vitro antioxidant activity assay methods and using the Kirby-Bauer disk diffusion susceptibility test, respectively. The elucidated antimicrobial and antioxidative activities of medical plant extracts were compared with data from the engineered biosynthetic AgNPs. The antimicrobial effect of engineered AgNPs against selected test cultures was found to be substantially stronger than for plant extracts used for their synthesis. The analysis of AgNPs by TEM revealed the presence of spherical-shaped nano-objects. The size distribution of AgNPs was found to be plant-type-dependent. The smaller AgNPs were obtained with extract (with a size range of 16.8 ± 5.8 nm compared to 35.7 ± 4.8 nm from AgNPs). The AgNPs' presumably inherited biological functions of and medical plants can provide a platform to combat pathogenic bacteria in the era of multi-drug resistance.