Multimodal Spectroscopic Imaging of Pea Root Nodules to Assess the Nitrogen Fixation in the Presence of Biofertilizer Based on Nod-Factors

• Published in: International journal of molecular sciences 2021-11, Vol.22 (23), p.12991
• Main Authors: Susniak, Katarzyna, Krysa, Mikolaj, Kidaj, Dominika, Szymanska-Chargot, Monika, Komaniecka, Iwona, Zamlynska, Katarzyna, Choma, Adam, Wielbo, Jerzy, Ilag, Leopold L, Sroka-Bartnicka, Anna
• Format: Article
• Language: English
• Subjects: Amino acids; Bacteria; Carbohydrates; Fertilizers; Fertilizers - microbiology; Flavonoids; Fourier transforms; FT-IR spectroscopy; Imaging; Infrared spectroscopy; Leghemoglobin - metabolism; Legumes; Lipids; MALDI MSI; Mass spectrometry; Mass spectroscopy; Metabolites; Morphology; Nitrogen; Nitrogen fixation; Nitrogen Fixation - physiology; Nitrogenation; Nod factor; Nod factors; Nodules; pea; Peas; Pisum sativum - growth & development; Pisum sativum - metabolism; Plant growth; Proteins; Raman spectroscopy; Rhizobium; Rhizobium leguminosarum - metabolism; Root nodules; Root Nodules, Plant - metabolism; Scientific imaging; Seeds; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Statistical analysis; symbiosis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222312991

Multimodal spectroscopic imaging methods such as Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI), Fourier Transform Infrared spectroscopy (FT-IR) and Raman spectroscopy were used to monitor the changes in distribution and to determine semi quantitatively selected metabolites involved in nitrogen fixation in pea root nodules. These approaches were used to evaluate the effectiveness of nitrogen fixation by pea plants treated with biofertilizer preparations containing Nod factors. To assess the effectiveness of biofertilizer, the fresh and dry masses of plants were determined. The biofertilizer was shown to be effective in enhancing the growth of the pea plants. In case of metabolic changes, the biofertilizer caused a change in the apparent distribution of the leghaemoglobin from the edges of the nodule to its centre (the active zone of nodule). Moreover, the enhanced nitrogen fixation and presumably the accelerated maturation form of the nodules were observed with the use of a biofertilizer.