Expression of DCX and Transcription Factor Profiling in Photothrombosis-Induced Focal Ischemia in Mice

Adult neurogenesis is present in the dentate gyrus and the subventricular zone in mammalian brain under physiological conditions. Recently, adult neurogenesis has also been reported in other brain regions after brain injury. In this study, we established a focal striatal ischemic model in adult mice...

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Published in:Frontiers in cellular neuroscience 2018-11, Vol.12, p.455-455
Main Authors: Lv, Zhu-Man, Zhao, Rong-Jian, Zhi, Xiao-Song, Huang, Ying, Chen, Jia-Yin, Song, Ning-Ning, Su, Chang-Jun, Ding, Yu-Qiang
Format: Article
Language:English
Subjects:
Astrocytes
Brain
Brain injury
Dentate gyrus
Doublecortin protein
Gene expression
Glial fibrillary acidic protein
Ischemia
Laboratories
Medical prognosis
Microglia
Neostriatum
Neurobiology
Neurogenesis
Neuroscience
Neurosciences
photothrombosis
Proteins
Rbp-J
Regeneration
Stem cells
striatum
Stroke
Subventricular zone
Transcription factors
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Summary:Adult neurogenesis is present in the dentate gyrus and the subventricular zone in mammalian brain under physiological conditions. Recently, adult neurogenesis has also been reported in other brain regions after brain injury. In this study, we established a focal striatal ischemic model in adult mice via photothrombosis (PT) and investigated how focal ischemia elicits neurogenesis in the striatum. We found that astrocytes and microglia increased in early post-ischemic stage, followed by a 1-week late-onset of doublecortin (DCX) expression in the striatum. The number of DCX-positive neurons reached the peak level at day 7, but they were still observed at day 28 post-ischemia. Moreover, Rbp-J (a key effector of Notch signaling) deletion in astrocytes has been reported to promote the neuron regeneration after brain ischemia, and we provided the change of gene expression profile in the striatum of astrocyte-specific Rbp-J knockout (KO) mice glial fibrillary acidic protein (GFAP-Cre :Rbp-J ), which may help to clarify detailed potential mechanisms for the post-ischemic neurogenesis in the striatum.
ISSN:1662-5102
1662-5102
DOI:10.3389/fncel.2018.00455