Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate from Ulmus pumila Leaves by GC-MS

Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different s...

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Bibliographic Details
Published in:International Journal of Analytical Chemistry 2015-01, Vol.2015 (2015), p.302-308
Main Authors: Gao, Bao-jia, Chen, Jian-xin, Wei, Dong, Shi, Bao-lin, Wang, Zhi-li, Huang, Zhi-hong, Wang, Su-li
Format: Article
Language:English
Subjects:
Acids
Analytical chemistry
Chemical properties
Gas chromatography
Infections
Jasmonates
Mass spectrometry
Methods
Nitrogen
Physiology
Rosidae
Salicylic acid
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Summary:Salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate are important phytohormones and defensive signaling compounds, so it is of great importance to determine their levels rapidly and accurately. The study uses Ulmus pumila leaves infected by Tetraneura akinire Sasaki at different stages as materials; after extraction with 80% methanol and ethyl acetate and purification with primary secondary amine (PSA) and graphitized carbon blacks (GCB), the contents of signal compounds salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate were determined by GC-MS. The results showed that the level of salicylic acid, jasmonic acid, methyl salicylate, and methyl jasmonate increased remarkably in U. pumila once infected by T. akinire Sasaki, but the maximums of these four compounds occurred at different times. Salicylic acid level reached the highest at the early stage, and jasmonic acid level went to the maximum in the middle stage; by contrast, change of content of methyl salicylate and methyl jasmonate was the quite opposite.
ISSN:1687-8760
1687-8779
DOI:10.1155/2015/698630