Effect of Lauric vs. Oleic Acid-Enriched Diets on Leptin Autoparacrine Signalling in Male Mice

High-fat diets enriched with lauric acid (SOLF) do not enhance leptin production despite expanding white adipose tissue (WAT). Our study aimed at identifying the influence of SOLF vs. oleic acid-enriched diets (UOLF) on the autoparacrine effect of leptin and was carried out on eight-week-old mice co...

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Published in:Biomedicines 2022-08, Vol.10 (8), p.1864
Main Authors: Fernández-Felipe, Jesús, Plaza, Adrián, Domínguez, Gema, Pérez-Castells, Javier, Cano, Victoria, Cioni, Francesco, Del Olmo, Nuria, Ruiz-Gayo, Mariano, Merino, Beatriz
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
Adipose tissue
adipose tissue hypertrophy
Adipose tissues
AKT protein
Analysis
Body fat
Care and treatment
Energy
Food
Food habits
Gene expression
Glycerol
Health aspects
High fat diet
Inflammation
Laboratory animals
Lauric acid
Leptin
leptin resistance
Lipids
Measurement
Metabolism
monounsaturated fatty acids
Morphology
NMR
Nuclear magnetic resonance
Oleic acid
perigonadal adipose tissue
Phosphorylation
saturated fatty acids
Signal transduction
Size distribution
Stat3 protein
subcutaneous adipose tissue
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Summary:High-fat diets enriched with lauric acid (SOLF) do not enhance leptin production despite expanding white adipose tissue (WAT). Our study aimed at identifying the influence of SOLF vs. oleic acid-enriched diets (UOLF) on the autoparacrine effect of leptin and was carried out on eight-week-old mice consuming control chow, UOLF or SOLF. Phosphorylation of kinases integral to leptin receptor (LepR) signalling pathways (705Tyr-STAT3, 473Ser-Akt, 172Thr-AMPK), adipocyte-size distribution, fatty acid content, and gene expression were analyzed in WAT. SOLF enhanced basal levels of phosphorylated proteins but reduced the ability of leptin to enhance kinase phosphorylation. In contrast, UOLF failed to increase basal levels of phosphorylated proteins and did not modify the effect of leptin. Both SOLF and UOLF similarly affected adipocyte-size distribution, and the expression of genes related with adipogenesis and inflammation. WAT composition was different between groups, with SOLF samples mostly containing palmitic, myristic and lauric acids (>48% w/w) and UOLF WAT containing more than 80% (w/w) of oleic acid. In conclusion, SOLF appears to be more detrimental than UOLF to the autoparacrine leptin actions, which may have an impact on WAT inflammation. The effect of SOLF and UOLF on WAT composition may affect WAT biophysical properties, which are able to condition LepR signaling.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines10081864