Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum

Wheat scab caused by is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as by whole genome annotation, 16S rRN...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2020-12, Vol.11, p.598285-598285
Main Authors: Xu, Wen, Zhang, Liyong, Goodwin, Paul H, Xia, Mingcong, Zhang, Jie, Wang, Qi, Liang, Juan, Sun, Runhong, Wu, Chao, Yang, Lirong
Format: Article
Language:English
Subjects:
Bacillus velezensis
biocontrol agent
DON
Fusarium graminearum
genome sequencing and assembly
Microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Wheat scab caused by is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as by whole genome annotation, 16S rRNA gene and average nucleotide identities analysis. The whole-genome sequence of strain YB-130 was obtained by PacBio sequencing. 88 putative Carbohydrate-Active Enzymes and 12 gene clusters encoding for secondary metabolites were identified in the YB-130 genome, including one gene cluster for the synthesis of lanthipeptide only found in strain YB-130 genome. In dual cultures, strain YB-130 significantly inhibited the growth of PH-1 and other eight fungal plant pathogens, indicating a broad antifungal activity. Furthermore, strain YB-130 was able to significantly inhibit spore morphology and hyphal development of PH-1. Strain YB-130 also reduced deoxynivalenol production by PH-1 in dual cultures, possibly due to its ability to suppress the expression of , , and that are required for deoxynivalenol production in . Overall, YB-130 is a promising biological control agent of both infection and mycotoxin production.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.598285