Comprehensive Genome-Wide Analysis of the Receptor-like Protein Gene Family and Functional Analysis of PeRLP8 Associated with Crown Rot Resistance in Passiflora edulis

• Published in: Plants (Basel) 2024-12, Vol.13 (23), p.3264
• Main Authors: Yu, Weijun, Liang, Fan, Li, Yue, Jiang, Wenjie, Li, Yongkang, Shen, Zitao, Fang, Ting, Zeng, Lihui
• Format: Article
• Language: English
• Subjects: Biological evolution; Biosynthesis; Breeding; Chromosomes; Crown rot; Cultivars; Disease resistance; Fruit rot; Fruits; Functional analysis; Fungal diseases; Gene expression; Genes; Genetic aspects; Genetic engineering; Genomes; Genomic analysis; Genomics; Immune response; Immunity (Disease); Kinases; Leaves; Low temperature; Molecular modelling; Oxygen; Passiflora edulis; Passion fruit; pathogen resistance; Pathogens; Pattern recognition; Phylogenetics; Plant breeding; Plant diseases; Plant immunity; Plant resistance; Proteins; Reactive oxygen species; receptor-like protein (RLP); Receptors; Rhizoctonia solani; Ribonucleic acid; RNA; Seedlings; Tobacco
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13233264

Passion fruit ( Sims) is a Passifloraceae plant with high economic value. Crown rot caused by is a major fungal disease, which can seriously reduce the yield and quality of passion fruit. Receptor-like proteins (RLPs), which act as pathogen recognition receptors, are widely involved in plant immune responses and developmental processes. However, the role of family members of passion fruit in resistance to crown rot remains unclear. In this study, evolutionary dynamics analysis and comprehensive genomic characterization of the genes family were performed on passion fruit. A total of 141 in the genome of the 'Zixiang' cultivar and 79 in the genome of the 'Tainong' cultivar were identified, respectively. Evolutionary analysis showed that proximal and dispersed duplication events were the primary drivers of family expansion. RNA-seq data and RT-qPCR analysis showed that were constitutively expressed in different tissues and induced by low temperature, JA, MeJA, and SA treatments. The gene was identified as the hub gene by RNA-seq analysis of passion fruit seedlings infected by . The expression levels of of the resistant variety (LG) were significantly higher than those of the sensitive variety f. (HG). Transient overexpression of tobacco and passion fruit leaves enhanced the resistance to , resulting in reduced lesion areas by 52.06% and 54.17%, respectively. In addition, it can increase reactive oxygen species levels and upregulated expression of genes related to active oxygen biosynthesis and JA metabolism in passion fruit leaves. Our research provides new insights into the molecular mechanism and breeding strategy of passion fruit resistance to crown rot.