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Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones
Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale. Our study aimed to...
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Published in: | Journal of applied oral science 2020-01, Vol.28, p.e20200242-e20200242 |
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container_title | Journal of applied oral science |
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creator | Saito, Miki Taketomi Mofatto, Luciana Souto Albiero, Mayra Laino Casati, Márcio Zafallon Sallum, Enilson Antonio Nociti Junior, Francisco Humberto SilvÉrio, Karina Gonzales |
description | Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale.
Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage.
To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes.
The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including "anatomical structure development" and "cell adhesion." Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones.
This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process "anatomical structure development," Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies. |
doi_str_mv | 10.1590/1678-7757-2020-0242 |
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Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage.
To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes.
The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including "anatomical structure development" and "cell adhesion." Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones.
This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process "anatomical structure development," Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies.</description><identifier>ISSN: 1678-7757</identifier><identifier>ISSN: 1678-7765</identifier><identifier>EISSN: 1678-7765</identifier><identifier>DOI: 10.1590/1678-7757-2020-0242</identifier><identifier>PMID: 33111882</identifier><language>eng</language><publisher>Brazil: Faculdade De Odontologia De Bauru - USP</publisher><subject>Adult ; Cadherins - metabolism ; Cell Differentiation ; Cells, Cultured ; Clone Cells ; Dental Cementum - cytology ; DENTISTRY, ORAL SURGERY & MEDICINE ; Humans ; Original ; Osteoblasts ; Osteoblasts - cytology ; Periodontal ligament ; Periodontal Ligament - cytology ; Sequence Analysis, RNA ; Transcriptome ; Wnt Signaling Pathway</subject><ispartof>Journal of applied oral science, 2020-01, Vol.28, p.e20200242-e20200242</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-79945c601b48669d5b7f7eb4c484e690176a97d2a9cc225fc86e005ae704e7eb3</citedby><cites>FETCH-LOGICAL-c510t-79945c601b48669d5b7f7eb4c484e690176a97d2a9cc225fc86e005ae704e7eb3</cites><orcidid>0000-0001-5879-9095</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648949/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648949/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,24150,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33111882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saito, Miki Taketomi</creatorcontrib><creatorcontrib>Mofatto, Luciana Souto</creatorcontrib><creatorcontrib>Albiero, Mayra Laino</creatorcontrib><creatorcontrib>Casati, Márcio Zafallon</creatorcontrib><creatorcontrib>Sallum, Enilson Antonio</creatorcontrib><creatorcontrib>Nociti Junior, Francisco Humberto</creatorcontrib><creatorcontrib>SilvÉrio, Karina Gonzales</creatorcontrib><title>Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones</title><title>Journal of applied oral science</title><addtitle>J Appl Oral Sci</addtitle><description>Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale.
Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage.
To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes.
The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including "anatomical structure development" and "cell adhesion." Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones.
This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process "anatomical structure development," Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies.</description><subject>Adult</subject><subject>Cadherins - metabolism</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Clone Cells</subject><subject>Dental Cementum - cytology</subject><subject>DENTISTRY, ORAL SURGERY & MEDICINE</subject><subject>Humans</subject><subject>Original</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Sequence Analysis, RNA</subject><subject>Transcriptome</subject><subject>Wnt Signaling Pathway</subject><issn>1678-7757</issn><issn>1678-7765</issn><issn>1678-7765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUstqHDEQFCEhdjb5gkCYYy5j6_24BILJw2DIIc4tIDSanl0tmtFEmjX476PJrpdYF0ndXdVFUQi9J_iKCIOviVS6VUqolmKKW0w5fYEuT1UpXp7fQl2gN6XsMWaUKfMaXTBGCNGaXqLf99lNxecwL2mEZs5pCBGaNDS7sN3FxyaVBVIXXVmCv_YwwrQ8fZsZckh9mhYXmxi2bm02HmJsfEwTlLfo1eBigXene4N-ff1yf_O9vfvx7fbm813rBcFLq4zhwktMOq6lNL3o1KCg455rDtJgoqQzqqfOeE-pGLyWgLFwoDCHOsg26PbI2ye3t3MOo8uPNrlg_xVS3lqXq-AIVvCBcQmcgGK819p0pueGOam6ATupK9fVkav4ADHZfTrkqYq3P1c37ermanc9BGOuVsCnI2A-dCP0vnqQXXym4nlnCju7TQ_WSK5N3b1BH08EOf05QFnsGMrqopsgHYqlXIhqgZC0jrLjqM-plAzDeQ3Bdg2FPYu0q0q7hqKiPvyv8Ix5SgH7C1MiscU</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Saito, Miki Taketomi</creator><creator>Mofatto, Luciana Souto</creator><creator>Albiero, Mayra Laino</creator><creator>Casati, Márcio Zafallon</creator><creator>Sallum, Enilson Antonio</creator><creator>Nociti Junior, Francisco Humberto</creator><creator>SilvÉrio, Karina Gonzales</creator><general>Faculdade De Odontologia De Bauru - USP</general><general>University of São Paulo</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5879-9095</orcidid></search><sort><creationdate>20200101</creationdate><title>Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones</title><author>Saito, Miki Taketomi ; Mofatto, Luciana Souto ; Albiero, Mayra Laino ; Casati, Márcio Zafallon ; Sallum, Enilson Antonio ; Nociti Junior, Francisco Humberto ; SilvÉrio, Karina Gonzales</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-79945c601b48669d5b7f7eb4c484e690176a97d2a9cc225fc86e005ae704e7eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Cadherins - metabolism</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Clone Cells</topic><topic>Dental Cementum - cytology</topic><topic>DENTISTRY, ORAL SURGERY & MEDICINE</topic><topic>Humans</topic><topic>Original</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Sequence Analysis, RNA</topic><topic>Transcriptome</topic><topic>Wnt Signaling Pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Miki Taketomi</creatorcontrib><creatorcontrib>Mofatto, Luciana Souto</creatorcontrib><creatorcontrib>Albiero, Mayra Laino</creatorcontrib><creatorcontrib>Casati, Márcio Zafallon</creatorcontrib><creatorcontrib>Sallum, Enilson Antonio</creatorcontrib><creatorcontrib>Nociti Junior, Francisco Humberto</creatorcontrib><creatorcontrib>SilvÉrio, Karina Gonzales</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of applied oral science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Miki Taketomi</au><au>Mofatto, Luciana Souto</au><au>Albiero, Mayra Laino</au><au>Casati, Márcio Zafallon</au><au>Sallum, Enilson Antonio</au><au>Nociti Junior, Francisco Humberto</au><au>SilvÉrio, Karina Gonzales</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones</atitle><jtitle>Journal of applied oral science</jtitle><addtitle>J Appl Oral Sci</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>28</volume><spage>e20200242</spage><epage>e20200242</epage><pages>e20200242-e20200242</pages><issn>1678-7757</issn><issn>1678-7765</issn><eissn>1678-7765</eissn><abstract>Heterogeneous cell populations of osteo/cementoblastic (O/C) or fibroblastic phenotypes constitute the periodontal dental ligament (PDL). A better understanding of these PDL cell subpopulations is essential to propose regenerative approaches based on a sound biological rationale.
Our study aimed to clarify the differential transcriptome profile of PDL cells poised to differentiate into the O/C cell lineage.
To characterize periodontal-derived cells with distinct differentiation capacities, single-cell-derived clones were isolated from adult human PDL progenitor cells and their potential to differentiate into osteo/cementoblastic (O/C) phenotype (C-O clones) or fibroblastic phenotype (C-F clones) was assessed in vitro. The transcriptome profile of the clonal cell lines in standard medium cultivation was evaluated using next-generation sequencing technology (RNA-seq). Over 230 differentially expressed genes (DEG) were identified, in which C-O clones showed a higher number of upregulated genes (193) and 42 downregulated genes.
The upregulated genes were associated with the Cadherin and Wnt signaling pathways as well as annotated biological processes, including "anatomical structure development" and "cell adhesion." Both transcriptome and RT-qPCR showed up-regulation of WNT2, WNT16, and WIF1 in C-O clones.
This comprehensive transcriptomic assessment of human PDL progenitor cells revealed that expression of transcripts related to the biological process "anatomical structure development," Cadherin signaling, and Wnt signaling can identify PDL cells with a higher potential to commit to the O/C phenotype. A better understanding of these pathways and their function in O/C differentiation will help to improve protocols for periodontal regenerative therapies.</abstract><cop>Brazil</cop><pub>Faculdade De Odontologia De Bauru - USP</pub><pmid>33111882</pmid><doi>10.1590/1678-7757-2020-0242</doi><orcidid>https://orcid.org/0000-0001-5879-9095</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adult Cadherins - metabolism Cell Differentiation Cells, Cultured Clone Cells Dental Cementum - cytology DENTISTRY, ORAL SURGERY & MEDICINE Humans Original Osteoblasts Osteoblasts - cytology Periodontal ligament Periodontal Ligament - cytology Sequence Analysis, RNA Transcriptome Wnt Signaling Pathway |
title | Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones |
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