Inhibition of retinal angiogenesis by gold nanoparticles via inducing autophagy

To investigate the effect of gold nanoparticles on retinal angiogenesis and , and to reveal the possible mechanism. Seed growth method was used to synthesize gold nanoparticles (GNPs). The size, zeta potential, absorption spectrum and morphology of GNPs were identified using Malvern Nano-ZS, multimo...

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Bibliographic Details
Published in:International journal of ophthalmology 2018-08, Vol.11 (8), p.1269-1276
Main Authors: Shen, Ni, Zhang, Rui, Zhang, Hao-Rui, Luo, Hao-Yang, Shen, Wei, Gao, Xin, Guo, Da-Zhi, Shen, Jie
Format: Article
Language:English
Subjects:
1276
autophagy
Basic Research
gold nanoparticles
nanomedicine
oxygen induced retinopathy
retinal angiogenesis
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Summary:To investigate the effect of gold nanoparticles on retinal angiogenesis and , and to reveal the possible mechanism. Seed growth method was used to synthesize gold nanoparticles (GNPs). The size, zeta potential, absorption spectrum and morphology of GNPs were identified using Malvern Nano-ZS, multimode reader (BioTek synergy2) and transmission electron microscope. Cell viability was analyzed using cell counting kit-8 method and cell growth was assessed with EdU kit. Transwell chamber was used to investigate cell migration. Tube formation method was used to assess the angiogenic property . Oxygen induced retinopathy (OIR) model was used to investigate the effect of GNPs on retinal angiogenesis. Confocal microscope and Western blot were used to study the possible mechanism of GNPs inhibited angiogenesis. The GNPs synthesized were uniform and well dispersed. GNPs of 10 µg/mL and 20 µg/mL were able to inhibit human umbilical vein endothelial cells proliferation (50% and 72% separately,
ISSN:2222-3959
2227-4898
DOI:10.18240/ijo.2018.08.04