Phospholipases Dα and δ are involved in local and systemic wound responses of cotton ( G. hirsutum )

• Published in: Biochemistry and biophysics reports 2017-03, Vol.9 (C), p.133-139
• Main Authors: Bourtsala, Angeliki, Farmaki, Theodora, Galanopoulou, Dia
• Format: Article
• Language: English
• Subjects: Cotton (Gossypium hirsutum); Phosphatidic acid; Phospholipase D activity; PLD substrates; PLDα and PLDδ; Wounding
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2016.12.001

Phospholipases D (PLDs) catabolize structural phospholipids to produce phosphatidic acid (PtdOH), a lipid playing central role in signalling pathways in animal, yeast and plant cells. In animal cells two PLD genes have been studied while in model plant twelve genes exist, classified in six classes (α-ζ). This underlines the role of these enzymes in plant responses to environmental stresses. However, information concerning the PLD involvement in the widely cultivated and economically important cotton plant responses is very limited. The aim of this report was to study the activity of conventional cotton PLD and its participation in plant responses to mechanical wounding, which resembles both biotic and abiotic stresses. PLDα activity was identified and further characterized by transphosphatidylation reaction. Upon wounding, cotton leaf responses consist of an acute increase of PLDα activity in both wounded and systemic tissue. However, determination of the PtdOH levels under the same wounding conditions revealed a rapid PtdOH formation only in wounded leaves and a late response of a PtdOH increase in both tissues. Εxpression analysis of PLDα and PLDδ isoforms showed mRNA accumulation of both isoforms in the wounded tissue, but only PLDδ exerts a high and sustainable expression in systemic leaves, indicating that this isoform is mainly responsible for the systemic wound-induced PtdOH production. Therefore, our data suggest that PLDα and PLDδ isoforms are involved in different steps in cotton wound signalling.