Anti-hyperglycemic fraction from Alternanthera sessilis L. leaves gets elucidated following bioassay-guided isolation and mass spectrometry

The anecdotal use of Alternanthera sessilis L. as a relief for diabetes has been known in the Philippines for generations, and antidiabetic activity of similar varieties in other countries is likewise documented. However, the compounds responsible for this activity remain unclear. This study aims to...

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Bibliographic Details
Published in:Brazilian Journal of Pharmaceutical Sciences 2023-01, Vol.59
Main Authors: Manalo, Richelle Ann Mallapre, Arollado, Erna Custodio, Heralde III, Francisco Maramara
Format: Article
Language:English
Subjects:
Alternanthera sessilis
Anti-hyperglycemic
Antidiabetics
Apigenin
Bioassay-guided isolation
Bioassays
Biodiversity
Chromatography
Cooperation
Coronaviruses
COVID-19
Diabetes
Disease
Expenditures
Glucose
Hyperglycemia
Internet
Luteolin
Mass spectrometry
Metabolism
PHARMACOLOGY & PHARMACY
Scientific imaging
Tan, Kim
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Summary:The anecdotal use of Alternanthera sessilis L. as a relief for diabetes has been known in the Philippines for generations, and antidiabetic activity of similar varieties in other countries is likewise documented. However, the compounds responsible for this activity remain unclear. This study aims to isolate the anti-hyperglycemic fraction of local A. sessilis leaves and identify the compounds in this fraction. Methanol extract of A. sessilis leaves and its hexane, ethyl acetate (ASE), and water fractions were administered to alloxan-induced diabetic mice. ASE (250mg/kg) had the highest anti-hyperglycemic activity at 6-h post-treatment (25.81%±12.72%), with almost similar blood glucose reduction rate as metformin (30.13±3.75%, p=0.767). Repeated fractionation employing chromatographic separation techniques followed by in vivo anti-hyperglycemic assay yielded partially purified subfractions. A. sessilis ethyl acetate subfraction 4-2 (100mg/kg) displayed remarkable suppression of blood glucose rise in diabetic mice at 6-h post-treatment (26.45±3.75%, p
ISSN:2175-9790
1984-8250
2175-9790
DOI:10.1590/s2175-97902023e21283