Data for semi-permanent cationic coating for protein separations

Protein separations and analyses are fundamental to fields of study that include biochemistry, biology, physiology, drug discovery, pharmaceuticals, as well as agricultural and food based industries. Here, we provide the data from a novel phospholipid-cetyltrimethylammonium bromide coating capable o...

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Bibliographic Details
Published in:Data in brief 2020-04, Vol.29, p.105123-105123, Article 105123
Main Authors: Crihfield, C.L., Kristoff, C.J., Veltri, L.M., Wilson, C.A., Penny, W.M., Holland, L.A.
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Chemistry
Electroosmotic flow
pH-stability
Protein adsorption
Semi-permanent coating
Surface modification
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Summary:Protein separations and analyses are fundamental to fields of study that include biochemistry, biology, physiology, drug discovery, pharmaceuticals, as well as agricultural and food based industries. Here, we provide the data from a novel phospholipid-cetyltrimethylammonium bromide coating capable of separating cationic and anionic proteins with high efficiency. Capillary electrophoresis separations of protein standards were utilized to characterize the performance of the novel coating. Using capillary electrophoresis with UV absorbance detection a working pH range of 4–9 was identified, with reproducibility in time ≤1% relative standard deviation, and plate counts for proteins as high as 480,000 plates (lysozyme, pH 7). Further details and results from these data are available in the work reported by Crihfield et al. and can be accessed at https://doi.org/10.1016/j.chroma.2019.460397 [1].
ISSN:2352-3409
2352-3409
DOI:10.1016/j.dib.2020.105123