Antidiabetic and Antioxidative Potential of the Blue Congo Variety of Purple Potato Extract in Streptozotocin-Induced Diabetic Rats

• Published in: Molecules (Basel, Switzerland) Switzerland), 2019-08, Vol.24 (17), p.3126
• Main Authors: Strugała, Paulina, Dzydzan, Olha, Brodyak, Iryna, Kucharska, Alicja Z, Kuropka, Piotr, Liuta, Mariana, Kaleta-Kuratewicz, Katarzyna, Przewodowska, Agnieszka, Michałowska, Dorota, Gabrielska, Janina, Sybirna, Natalia
• Format: Article
• Language: English
• Subjects: Advanced glycosylation end products; Animals; Anthocyanins; Anthocyanins - administration & dosage; Anthocyanins - chemistry; Anthocyanins - pharmacology; antidiabetic activity; Antidiabetics; antioxidant enzymes; Antioxidants - administration & dosage; Antioxidants - chemistry; Antioxidants - pharmacology; blood; Blood Glucose - analysis; Blood Glucose - drug effects; Blue Congo; Carbonyl compounds; Carbonyls; Chromatography, High Pressure Liquid; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; DNA methylation; Free radicals; Fruits; Glucose; Glycated Hemoglobin A - analysis; Glycated Hemoglobin A - drug effects; Glycosylation; Hydroxybenzoates - administration & dosage; Hydroxybenzoates - chemistry; Hydroxybenzoates - pharmacology; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Lipid peroxidation; Lipids; Male; markers of oxidative stress; Oxidation; Oxidative stress; Peroxidation; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - pharmacology; Potatoes; Proteins; purple potatoes; Rats; Rats, Wistar; Rodents; Scavenging; Solanum tuberosum - chemistry; Streptozocin; streptozotocin-induced diabetes mellitus
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24173126

This study was designed to evaluate the effects of purple potato extract of the Blue Congo variety (PP) on diabetes and its antioxidant activities after two-week administration tostreptozotocin (STZ)-induced diabetic rats. The activities of PP were evaluated at a dose of 165 mg/kg body weight (b.w.) by estimating biochemical changes in blood plasma and through a histopathological study of kidney, muscles, and liver tissue. We evaluated the effect of treatment with extract on glucose level, glycated hemoglobin, activities of enzymatic antioxidants (including superoxide dismutase, glutathione peroxidase, and catalase), and lipid peroxidation. Moreover, we determined advanced glycation end-products (AGEs), advanced oxidation protein products (AOPPs), and the level of oxidative modified proteins (OMPs) as markers of carbonyl-oxidative stress in rats with diabetes. Using high-performance liquid chromatography, we identified five anthocyanins and six phenolic acids in the extract from Blue Congo with the dominant acylated anthocyanin as petunidin-3- -coumaroyl-rutinoside-5-glucoside. The administration of Blue Congo extract lowered blood glucose, improved glucose tolerance, and decreased the amount of glycated hemoglobin. Furthermore, PP demonstrated an antioxidative effect, suppressed malondialdehyde levels, and restored antioxidant enzyme activities in diabetic rats. After administration of PP, we also noticed inhibition of OMP, AGE, and AOPP formation in the rats' blood plasma.