Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers

Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2020-07, Vol.9 (8), p.1788
Main Authors: Reid, Paul, Staudacher, Alexander H, Marcu, Loredana G, Olver, Ian, Moghaddasi, Leyla, Brown, Michael P, Li, Yanrui, Bezak, Eva
Format: Article
Language:English
Subjects:
Care and treatment
Cell culture
Cell lines
clonogenic assay
Comparative analysis
Complications and side effects
fractionated radiation therapy
Fractionation
Generations
Head & neck cancer
Head and neck
Head and neck cancer
Head and neck carcinoma
Head and Neck Neoplasms - virology
HNSCCC
HPV
Human papillomavirus
Humans
Papillomavirus infections
Papillomavirus Infections - radiotherapy
Radiation therapy
Radiation Tolerance
Radiosensitivity
Software
Squamous cell carcinoma
Tobacco
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Summary:Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV status, in their response to radiation dose. Previously unexamined changes in radio-responsiveness following fractionated X-ray irradiation were compared between HPV positive and negative statuses of HNSCC. Six HNSCC cell lines, 3 of each HPV status, were investigated for radiosensitivity by clonogenic assay and modelled by response as a function of dose. Generational cultures of each cell line were developed to follow changes in radiosensitivity after repeated irradiations simulating fractionated radiation therapy. As a group, the HPV positive cell lines were more radiosensitive, but with changes following repeated fractions of dose, and modelling of response as a function of dose, both statuses displayed large radiobiological heterogeneity. These findings challenge current radiobiological assumptions of head and neck cancers as early responding tissue to radiation and may go some way in explaining difficulties reaching consensus in stratification of treatment by HPV status. Consequently, results from this study do not support stratifying radiation therapy by HPV status.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9081788