Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis

Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide. Growing studies have proofed that circular RNAs (circRNAs) engage in the regulation of various types of cancers. However, the underlying biological functions and effect mechanism of circular RNA_LARP4 (cir...

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Bibliographic Details
Published in:Cancer cell international 2020-01, Vol.20 (1), p.5-5, Article 5
Main Authors: Lin, Wumei, Ye, Haiyan, You, Keli, Chen, Le
Format: Article
Language:English
Subjects:
Assaying
Cancer therapies
Cell adhesion & migration
Cell growth
Cell migration
Cell proliferation
ceRNA
Cholecystokinin
circ_LARP4
Circular RNA
Cloning
Epithelial cells
Gastric cancer
Gene expression
Immunoprecipitation
LARP4
Medical prognosis
MicroRNAs
miR-513b-5p
Ovarian cancer
Polymerase chain reaction
Primary Research
Proteins
Reproductive system
Ribonucleic acid
RNA
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Summary:Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide. Growing studies have proofed that circular RNAs (circRNAs) engage in the regulation of various types of cancers. However, the underlying biological functions and effect mechanism of circular RNA_LARP4 (circ_LARP4) in OC have not been explored. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to detect the expression of circ_LARP4 in OC cells. The function of circ_LARP4 was measured by cell counting kit-8 (CCK-8), colony formation assay and transwell assay. RNA immunoprecipitation (RIP) assay and luciferase reporter assays assessed the binding correlation between miR-513b-5p and circ_LARP4 (or LARP4). The expression of circ_LARP4 in OC cells was much lower than that in human normal ovarian epithelial cells. Overexpressing circ_LARP4 impaired cell proliferation, invasion and migration abilities. Circ_LARP4 worked as a competing endogenous RNA (ceRNA) to sponge miR-513b-5p. Furthermore, LARP4 was indirectly modulated by circ_LARP4 as the downstream target of miR-513b-5p, as well as the host gene of circ_LARP4. Circ_LARP4 could hamper cell proliferation and migration by sponging miR-513b-5p to regulate the expression of LARP4. This research may provide some referential value to OC treatment.
ISSN:1475-2867
1475-2867
DOI:10.1186/s12935-019-1071-z