A novel method to augment extraction of mangiferin by application of microwave on three phase partitioning

•Report novel technique for mangiferin extraction from Mangifera indica leaves.•MTTP gives higher yield as compared with other conventional and novel techniques.•It gives simultaneous extraction and purification of biomolecules in a short time.•It can be used as a basis for separation of other molec...

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Bibliographic Details
Published in:Biotechnology reports (Amsterdam, Netherlands) Netherlands), 2015-06, Vol.6 (C), p.8-12
Main Authors: Kulkarni, Vrushali M., Rathod, Virendra K.
Format: Article
Language:English
Subjects:
Extraction
Mangifera indica
Mangiferin
Microwave
Three phase partitioning
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Summary:•Report novel technique for mangiferin extraction from Mangifera indica leaves.•MTTP gives higher yield as compared with other conventional and novel techniques.•It gives simultaneous extraction and purification of biomolecules in a short time.•It can be used as a basis for separation of other molecules from natural source. This work reports a novel approach where three phase partitioning (TPP) was combined with microwave for extraction of mangiferin from leaves of Mangifera indica. Soxhlet extraction was used as reference method, which yielded 57mg/g in 5h. Under optimal conditions such as microwave irradiation time 5min, ammonium sulphate concentration 40% w/v, power 272W, solute to solvent ratio 1:20, slurry to t-butanol ratio 1:1, soaking time 5min and duty cycle 50%, the mangiferin yield obtained was 54mg/g by microwave assisted three phase partitioning extraction (MTPP). Thus extraction method developed resulted into higher extraction yield in a shorter span, thereby making it an interesting alternative prior to down-stream processing.
ISSN:2215-017X
2215-017X
DOI:10.1016/j.btre.2014.12.009