Unveiling hsa_circ_0007439: a novel suppressor of nasopharyngeal carcinoma via miR-556-5p/PTEN Axis

Objective There has been no explanation for the exact mechanism of circRNAs in nasopharyngeal carcinoma (NPC). Circ_0007439 was investigated in this study to determine its role and mechanism in NPC. Methods To identify circ_0007439, microRNA (miR)-556-5p, and PTEN mRNA in NPC tissues and cells, RT-q...

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Bibliographic Details
Published in:Discover. Oncology 2024-12, Vol.15 (1), p.807-12
Main Authors: Li, Ming, Yang, Jing, Feng, WanRong, Fu, DongXue, Bai, Yang
Format: Article
Language:English
Subjects:
Apoptosis
Bioinformatics
Cancer
Cancer Research
Cell growth
Circ_0007439
Datasets
Flow cytometry
Genes
Internal Medicine
Medicine
Medicine & Public Health
MicroRNAs
MiR-556-5p
Molecular Medicine
Nasopharyngeal carcinoma
Oncology
Phosphatase
Proliferation
Proteins
PTEN
Radiation therapy
Radiotherapy
Software
Statistical analysis
Surgical Oncology
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Summary:Objective There has been no explanation for the exact mechanism of circRNAs in nasopharyngeal carcinoma (NPC). Circ_0007439 was investigated in this study to determine its role and mechanism in NPC. Methods To identify circ_0007439, microRNA (miR)-556-5p, and PTEN mRNA in NPC tissues and cells, RT-qPCR was employed. PTEN protein was quantified using western blot analysis. The impact of circ_0007439 on cellular activities was assessed using CCK-8, colony formation, Transwell, and flow cytometry, in that order. The interaction of miR-556-5p with circ_0007439 or PTEN mRNA was confirmed through a dual luciferase reporter gene assay and RIP assay. Results circ_0007439 and PTEN levels were low in NPC tissues and cells, while miR-556-5p was highly expressed. NPC cells were inhibited in proliferation, migration, invasion, and anti-apoptosis when circ_0007439 was upregulated or miR-556-5p was reduced. The circ_0007439 molecule acted as a molecular sponge that inhibited miR-556-5p’s suppression of PTEN expression. Additionally, reduction of PTEN weakened the inhibitive effects of elevating circ_0007439 or reducing miR-556-5p on NPC cells. Conclusion Circ_0007439 mediates miR-556-5p/PTEN axis to inhibit NPC progression as an potential target for NPC therapy.
ISSN:2730-6011
2730-6011
DOI:10.1007/s12672-024-01656-z