Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates

A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglyc...

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Bibliographic Details
Published in:Journal of lipid research 1982-05, Vol.23 (4), p.660-663
Main Authors: Fine, J B, Sprecher, H
Format: Article
Language:English
Subjects:
Animals
Blood Platelets - analysis
Boric Acids
Chemical Phenomena
Chemistry
Chromatography, Thin Layer - methods
Kidney - analysis
Liver - analysis
Phospholipids - isolation & purification
Rats
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Summary:A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol. Liver, kidney, and platelet phospholipids of the rat were resolved into six major classes using this method. Each was judged to be pure by two-dimensional thin-layer chromatography. The use of boric acid thin-layer chromatography for the separation of phospholipids should find wide application.
ISSN:0022-2275
DOI:10.1016/s0022-2275(20)38132-3