Biochemically Programmable Isothermal PCR

Isothermal PCR can be performed by imposing a static temperature gradient that continuously circulates reagents through denaturing, annealing, and extension conditions inside a PCR tube. But despite early promise, these systems have yet to demonstrate performance and repeatability sufficient for ado...

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Bibliographic Details
Published in:Advanced science 2024-11, Vol.11 (41), p.e2404688-n/a
Main Authors: Kim, MinGin, Ravisankar, Vijay, Hassan, Yassin A., Ugaz, Victor M.
Format: Article
Language:English
Subjects:
biochemistry
DNA - genetics
DNA Primers - genetics
Flow velocity
Geometry
nucleic acid analysis
PCR
point of care diagnostics
Polymerase Chain Reaction - methods
Reagents
Reproducibility of Results
Temperature
Thermal cycling
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Summary:Isothermal PCR can be performed by imposing a static temperature gradient that continuously circulates reagents through denaturing, annealing, and extension conditions inside a PCR tube. But despite early promise, these systems have yet to demonstrate performance and repeatability sufficient for adoption in validated laboratory tests because the rate‐limiting extension step is inherently short and cannot be increased independently of the other stages in a temperature cycle. Here, a discovery that enables isothermal PCR to be achieved with statistically robust repeatability that meets or exceeds diagnostic assay requirements (false positive/negative rate
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202404688