The Study of Performance of a Nanoribbon Biosensor, Sensitized with Aptamers and Antibodies, upon Detection of Core Antigen of Hepatitis C Virus

The development of highly sensitive diagnostic systems for the early revelation of diseases in humans is one of the most important tasks of modern biomedical research, and the detection of the core antigen of the hepatitis C virus (HCVcoreAg)-a protein marker of the hepatitis C virus-is just the cas...

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Bibliographic Details
Published in:Micromachines (Basel) 2023-10, Vol.14 (10), p.1946
Main Authors: Ivanov, Yuri D, Malsagova, Kristina A, Goldaeva, Kristina V, Pleshakova, Tatyana O, Kozlov, Andrey F, Galiullin, Rafael A, Shumov, Ivan D, Popov, Vladimir P, Abramova, Irina K, Ziborov, Vadim S, Petrov, Oleg F, Dolgoborodov, Alexander Yu, Archakov, Alexander I
Format: Article
Language:English
Subjects:
Antibodies
antibody
Antigens
aptamer
Biological products
Biomarkers
Biosensors
Detectors
Diagnostic systems
diagnostics
Galactosidase
HCVcoreAg
Hepatitis C
Hepatitis C virus
Infections
nanoribbon biosensor
Nanoribbons
Potassium
Proteins
Sensors
Viral antibodies
Viral infections
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Summary:The development of highly sensitive diagnostic systems for the early revelation of diseases in humans is one of the most important tasks of modern biomedical research, and the detection of the core antigen of the hepatitis C virus (HCVcoreAg)-a protein marker of the hepatitis C virus-is just the case. Our study is aimed at testing the performance of the nanoribbon biosensor in the case of the use of two different types of molecular probes: the antibodies and the aptamers against HCVcoreAg. The nanoribbon sensor chips employed are based on "silicon-on-insulator structures" (SOI-NR). Two different HCVcoreAg preparations are tested: recombinant β-galactosidase-conjugated HCVcoreAg ("Virogen", Watertown, MA, USA) and recombinant HCVcoreAg ("Vector-Best", Novosibirsk, Russia). Upon the detection of either type of antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 was found to be approximately equal, amounting to ~10 M. This value was similar upon the use of either type of molecular probes.
ISSN:2072-666X
2072-666X
DOI:10.3390/mi14101946