Elucidating the Host Interactome of EV-A71 2C Reveals Viral Dependency Factors

Viral protein 2C plays a critical role in EV-A71 replication. The discovery of 2C binding proteins will likely provide potential targets to treat EV-A71 infection. Here, we provide a global proteomic analysis of the human proteins that interact with the EV-A71 2C protein. TRIM4, exportin2, and ARFGA...

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Bibliographic Details
Published in:Frontiers in microbiology 2019-04, Vol.10, p.636-636
Main Authors: Li, Ye, Jian, Xia, Yin, Peiqi, Zhu, Guofeng, Zhang, Leiliang
Format: Article
Language:English
Subjects:
ARFGAP1
EV-A71
exportin2
Microbiology
TRIM4
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Summary:Viral protein 2C plays a critical role in EV-A71 replication. The discovery of 2C binding proteins will likely provide potential targets to treat EV-A71 infection. Here, we provide a global proteomic analysis of the human proteins that interact with the EV-A71 2C protein. TRIM4, exportin2, and ARFGAP1 were validated as 2C binding partners. Further functional studies revealed that TRIM4, exportin2, and ARFGAP1 were novel host dependency factors for EV-A71. Moreover, enteroviruses' 2C family proteins interacted with exportin2 and ARFGAP1. In conclusion, our study provides a cellular interactome of the EV-A71 2C and identifies the proviral roles of TRIM4, exportin2, and ARFGAP1 in EV-A71 infection.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.00636