Screening for Astragalus hamosus Triterpenoid Saponins Using HPTLC Methods: Prior Identification of Azukisaponin Isomers

Due to their particular structural characteristics, the extraction and isolation of saponins from plants present a serious challenge. In this study, specific extraction protocols were first implemented to extract the secondary metabolites from and, more precisely, the saponins. Subsequent purificati...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2022-08, Vol.27 (17), p.5376
Main Authors: Nafti, Khouloud, Giacinti, Geraldine, Marghali, Sonia, Raynaud, Christine Delgado
Format: Article
Language:English
Subjects:
Alzheimer's disease
Astragalus hamosus
Astragalus Plant - chemistry
azukisaponin
Biological activity
Breast cancer
Chromatography
Chromatography, Thin Layer - methods
Development systems
HPTLC
Isomers
Life Sciences
Metabolites
Plant Extracts - chemistry
Polarity
Purification
Saponins
Saponins - chemistry
Secondary metabolites
Seeds
Solvents
soyasaponin
Thin layer chromatography
Triterpenes - chemistry
triterpenoid saponin
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Summary:Due to their particular structural characteristics, the extraction and isolation of saponins from plants present a serious challenge. In this study, specific extraction protocols were first implemented to extract the secondary metabolites from and, more precisely, the saponins. Subsequent purification of the extracts was based on a single chromatographic technique, high-performance thin-layer chromatography, applying two development systems: a one-step system that separated molecules according to their polarity and a multiple development system that made it possible to detect the triterpenoid saponins, azukisaponin or soyasapogenol at a retarded Rf of 0.2. The difficulties of detecting the saponins encountered during the extraction and purification of the extracts have been highlighted and the strategy carried out to isolate the saponins has been discussed.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27175376