A rapid, isocratic method for phospholipid separation by high-performance liquid chromatography

A rapid, isocratic method for separating the most prevalent phospholipids by high-performance liquid chromatography is described. Baseline resolution of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine, and sphingomyelin is achieved in...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 1983-10, Vol.24 (10), p.1398-1403
Main Authors: Kaduce, T L, Norton, K C, Spector, A A
Format: Article
Language:English
Subjects:
Animals
Aorta - analysis
Cattle
Chromatography, High Pressure Liquid - methods
Endothelium - analysis
Fatty Acids - analysis
Glycine max
Muscle, Smooth, Vascular - analysis
Phosphatidylcholines - isolation & purification
Phospholipids - isolation & purification
Plants - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A rapid, isocratic method for separating the most prevalent phospholipids by high-performance liquid chromatography is described. Baseline resolution of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine, and sphingomyelin is achieved in less than 40 min on a silica column. Lipids are injected in 10 microliter of chloroform-diethyl ether 1:2 (v/v) and eluted with a solvent mixture of acetonitrile-methanol-sulfuric acid 100:3:0.05 (v/v/v) at a flow rate of 1 ml/min. Neutral lipids and cardiolipin elute with the solvent front. Chromatography of a radioactive cell lipid extract indicates a recovery of better than 97%. The procedure is sensitive enough to permit the analysis of the main phospholipids present in a monolayer culture containing about 100 micrograms of cell protein.
ISSN:0022-2275
DOI:10.1016/s0022-2275(20)37891-3