Diverse Fluoroquinolone Resistance Plasmids From Retail Meat E. coli in the United States

Fluoroquinolones are used to treat serious bacterial infections, including those caused by and . The emergence of plasmid-mediated quinolone resistance (PMQR) represent a new challenge to the successful treatment of Gram-negative infections. As part of a long-term strategy to generate a reference da...

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Bibliographic Details
Published in:Frontiers in microbiology 2019-12, Vol.10, p.2826
Main Authors: Tyson, Gregory H, Li, Cong, Hsu, Chih-Hao, Bodeis-Jones, Sonya, McDermott, Patrick F
Format: Article
Language:English
Subjects:
antimicrobial resistance
Escherichia coli
fluoroquinolones
Microbiology
PacBio
plasmids
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Summary:Fluoroquinolones are used to treat serious bacterial infections, including those caused by and . The emergence of plasmid-mediated quinolone resistance (PMQR) represent a new challenge to the successful treatment of Gram-negative infections. As part of a long-term strategy to generate a reference database of closed plasmids from antimicrobial resistant foodborne bacteria, we performed long-read sequencing of 11 isolates from retail meats that were non-susceptible to ciprofloxacin. Each of the isolates had PMQR genes, including , , and . The four genes were carried on two distinct ColE-type plasmids among isolates from pork chop and ground turkey and were identical to plasmids previously identified in . Seven other plasmids differed from any other sequences in GenBank and comprised IncF and IncR plasmids that ranged in size from 48 to 180 kb. These plasmids also contained different combinations of resistance genes, including those conferring resistance to beta-lactams, macrolides, sulfonamides, tetracycline, and heavy metals. Although relatively few isolates have PMQR genes, the identification of diverse plasmids in multiple retail meat sources suggests the potential for further spread of fluoroquinolone resistance, including through co-selection. These results highlight the value of long-read sequencing in characterizing antimicrobial resistance genes of public health concern.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.02826