Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA

The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein⁻DNA interactions. Imaging methods using small mole...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-01, Vol.24 (3), p.491
Main Authors: Kaur, Gurleen, Lewis, Jacob S, van Oijen, Antoine M
Format: Article
Language:English
Subjects:
Biophysical Phenomena
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - genetics
DNA - ultrastructure
DNA complexes
DNA probes
DNA Replication
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - genetics
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Dyes
Dynamic mechanical properties
fluorescence microscopy
Fluorescent Dyes
Free radicals
Mechanical properties
Microscopy
Microscopy, Fluorescence
Nucleic acids
Polymers
Probes
protein complexes
Proteins
Review
Sequence Analysis, DNA
Single Molecule Imaging - methods
single molecules
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Summary:The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein⁻DNA interactions. Imaging methods using small molecules and protein-based probes to visualise DNA have propelled our understanding of complex biochemical reactions involving DNA. This review focuses on summarising some of the methodological developments made to visualise individual DNA molecules and discusses how these probes have been used in single-molecule biophysical assays.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24030491