Characterization and bioactivity of polysaccharides obtained from pine cones of Pinus koraiensis by graded ethanol precipitation

Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and a...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2013-08, Vol.18 (8), p.9933-9948
Main Authors: Zou, Pan, Yang, Xin, Huang, Wei-Wei, Zhao, Hai-Tian, Wang, Jing, Xu, Ren-Bo, Hu, Xing-Long, Shen, Si-Yan, Qin, Di
Format: Article
Language:English
Subjects:
Arabinose - chemistry
bioactivity
Biological activity
chemical characterization
Chromatography, High Pressure Liquid
Ethanol
Ethanol - chemistry
Galactose - chemistry
Gas Chromatography-Mass Spectrometry
Glucose - chemistry
graded ethanol precipitation
Mannose - chemistry
Molecular weight
pine cones
Pinus - chemistry
Pinus koraiensis
polysaccharides
Polysaccharides - chemistry
Proteins
Ribose - chemistry
Spectroscopy, Fourier Transform Infrared
Xylose - chemistry
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Summary:Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of D-ribose, L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose and D-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg · mL-1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules18089933