Molecular identification and antifungal susceptibility profile of Candida species isolated from patients with vulvovaginitis in Tehran, Iran

Background: Rapid and accurate identification and evaluation of antifungal susceptibility pattern of Candida isolates are crucial to determine suitable antifungal drugs for the treatment of patients with vulvovaginitis candidiasis. Materials and Methods: Vaginal samples were collected from 150 women...

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Bibliographic Details
Published in:Journal of research in medical sciences 2017-01, Vol.22 (1), p.132-132
Main Authors: Sharifynia, Somayeh, Falahati, Mehraban, Akhlaghi, Lame, Foroumadi, Alireza, Fateh, Roohollah
Format: Article
Language:English
Subjects:
Azolic antifungal drugs
Candida species
Drugs
Enzymes
Epidemiology
Gynecology
Identification
Infections
Methods
Obstetrics
Parasitology
Patients
Polymerase chain reaction
polymerase chain reaction-restriction fragment length polymorphism
Research centers
Short Communication
Tuberculosis
vulvovaginitis candidiasis
Yeast
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Summary:Background: Rapid and accurate identification and evaluation of antifungal susceptibility pattern of Candida isolates are crucial to determine suitable antifungal drugs for the treatment of patients with vulvovaginitis candidiasis. Materials and Methods: Vaginal samples were collected from 150 women with suspicious vaginal candidiasis, and then cultured on Sabouraoud's Dextrose Agar with chloramphenicol to isolate Candida species. After identification of Candida isolates using polymerase chain reaction-restriction fragment length polymorphism technique, antifungal susceptibility testing of four azolic antifungal drugs was carried out using broth microdilution method according to the CLSI M27-A3. Results: Candida species were isolated from eighty suspected patients (61.79%). The most common pathogen was Candida albicans (63.75%). Resistance to fluconazole and ketoconazole was observed in 27.5% and 23.75% of Candida isolates, respectively, and only 2% of Candida isolates were resistant to miconazole. Interestingly, resistance to fluconazole in C. albicans was more than other Candida species. Conclusion: The results indicated that therapy should be selected according to the antifungal susceptibility tests for the prevention of treatment failure and miconazole therapy can be considered as the best therapeutic choice in the management of vulvovaginitis.
ISSN:1735-1995
1735-1995
1735-7136
DOI:10.4103/jrms.JRMS_106_17