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Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC) from Southern Alberta, Canada, from 2020 to 2022
Shiga toxin-producing (STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all c...
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| Published in: | Pathogens (Basel) 2024-09, Vol.13 (10), p.822 |
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| cites | cdi_FETCH-LOGICAL-c443t-fb2330cec7bfb822d475530f67d448a7850c3d3b4936f6c9e9a9d0fbcaa46a5b3 |
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| container_issue | 10 |
| container_start_page | 822 |
| container_title | Pathogens (Basel) |
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| creator | Glassman, Heather Suttorp, Vivien White, Theron Ziebell, Kim Kearney, Ashley Bessonov, Kyrylo Li, Vincent Chui, Linda |
| description | Shiga toxin-producing
(STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including
,
, and
, were more frequent in HUS cases. The number of samples was too low to determine statistical significance. |
| doi_str_mv | 10.3390/pathogens13100822 |
| format | article |
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(STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including
,
, and
, were more frequent in HUS cases. The number of samples was too low to determine statistical significance.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens13100822</identifier><identifier>PMID: 39452694</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Age ; Causes of ; Children ; Clinical isolates ; Clinical outcomes ; Cluster analysis ; Communication ; Demographic aspects ; DNA sequencing ; E coli ; Escherichia coli ; Escherichia coli infections ; Gene sequencing ; Genetic aspects ; Genomes ; Hospitalization ; Identification and classification ; Infections ; Inflammatory bowel disease ; Kidney diseases ; Laboratories ; Microbiological research ; Nucleotide sequencing ; Patient outcomes ; Patients ; Serotypes ; Shiga toxin ; Shiga toxin-producing Escherichia coli ; Statistical analysis ; Surveillance ; Toxins ; Virulence ; Virulence (Microbiology) ; Virulence factors ; Whole genome sequencing</subject><ispartof>Pathogens (Basel), 2024-09, Vol.13 (10), p.822</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c443t-fb2330cec7bfb822d475530f67d448a7850c3d3b4936f6c9e9a9d0fbcaa46a5b3</cites><orcidid>0000-0003-0851-9803</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3120701673/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3120701673?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53770,53772,74873</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39452694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Glassman, Heather</creatorcontrib><creatorcontrib>Suttorp, Vivien</creatorcontrib><creatorcontrib>White, Theron</creatorcontrib><creatorcontrib>Ziebell, Kim</creatorcontrib><creatorcontrib>Kearney, Ashley</creatorcontrib><creatorcontrib>Bessonov, Kyrylo</creatorcontrib><creatorcontrib>Li, Vincent</creatorcontrib><creatorcontrib>Chui, Linda</creatorcontrib><title>Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC) from Southern Alberta, Canada, from 2020 to 2022</title><title>Pathogens (Basel)</title><addtitle>Pathogens</addtitle><description>Shiga toxin-producing
(STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including
,
, and
, were more frequent in HUS cases. 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(STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including
,
, and
, were more frequent in HUS cases. The number of samples was too low to determine statistical significance.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39452694</pmid><doi>10.3390/pathogens13100822</doi><orcidid>https://orcid.org/0000-0003-0851-9803</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2076-0817 |
| ispartof | Pathogens (Basel), 2024-09, Vol.13 (10), p.822 |
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| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_5668818fbb174d1eba403d9ec98835a2 |
| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Age Causes of Children Clinical isolates Clinical outcomes Cluster analysis Communication Demographic aspects DNA sequencing E coli Escherichia coli Escherichia coli infections Gene sequencing Genetic aspects Genomes Hospitalization Identification and classification Infections Inflammatory bowel disease Kidney diseases Laboratories Microbiological research Nucleotide sequencing Patient outcomes Patients Serotypes Shiga toxin Shiga toxin-producing Escherichia coli Statistical analysis Surveillance Toxins Virulence Virulence (Microbiology) Virulence factors Whole genome sequencing |
| title | Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC) from Southern Alberta, Canada, from 2020 to 2022 |
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