PP1-Mediated Dephosphorylation of Lgl Controls Apical-basal Polarity

• Published in: Cell reports (Cambridge) 2019-01, Vol.26 (2), p.293-301.e7
• Main Authors: Moreira, Sofia, Osswald, Mariana, Ventura, Guilherme, Gonçalves, Margarida, Sunkel, Claudio E., Morais-de-Sá, Eurico
• Format: Article
• Language: English
• Subjects: Animals; aPKC; Aurora Kinase A - metabolism; Binding Sites; cell division; Cell Polarity; Drosophila; Drosophila melanogaster; Drosophila Proteins - chemistry; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Epithelial Cells - metabolism; epithelial tissue; Lgl; live imaging; Mitosis; phosphorylation; Protein Binding; protein phosphatase 1; Protein Phosphatase 1 - metabolism; Protein Transport; Tumor Suppressor Proteins - chemistry; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2018.12.060

Apical-basal polarity is a common trait that underlies epithelial function. Although the asymmetric distribution of cortical polarity proteins works in a functioning equilibrium, it also retains plasticity to accommodate cell division, during which the basolateral determinant Lgl is released from the cortex. Here, we investigated how Lgl restores its cortical localization to maintain the integrity of dividing epithelia. We show that cytoplasmic Lgl is reloaded to the cortex at mitotic exit in Drosophila epithelia. Lgl cortical localization depends on protein phosphatase 1, which dephosphorylates Lgl on the serines phosphorylated by aPKC and Aurora A kinases through a mechanism that relies on the regulatory subunit Sds22 and a PP1-interacting RVxF motif of Lgl. This mechanism maintains epithelial polarity and is of particular importance at mitotic exit to couple Lgl cortical reloading with the polarization of the apical domain. Hence, PP1-mediated dephosphorylation of Lgl preserves the apical-basal organization of proliferative epithelia. [Display omitted] •Cytoplasmic Lgl returns to the cortex at mitotic exit in Drosophila epithelia•Lgl cortical localization depends on PP1-mediated dephosphorylation•PP1 dephosphorylates Lgl to maintain epithelial architecture•Apical polarization of new daughter cells is coupled with Lgl cortical reloading Moreira et al. investigate the post-mitotic polarization of new daughter cells in Drosophila epithelia. They identify PP1 phosphatase as a regulator of the conserved basolateral determinant Lgl. Dephosphorylation of Lgl counteracts aPKC/AurA activity, being an essential mechanism to restore Lgl cortical localization and to maintain the architecture of proliferative tissue.