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Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films
The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na 2 CO 3 an...
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| Published in: | Journal of natural fibers 2022-11, Vol.19 (15), p.11114-11123 |
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| container_end_page | 11123 |
| container_issue | 15 |
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| container_title | Journal of natural fibers |
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| creator | Yang, Hui Guo, Yuhang Zhang, Qiang Yan, Shuqin You, Renchuan |
| description | The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na
2
CO
3
and NaHCO
3
are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO
3
degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na
2
CO
3
degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na
2
CO
3
degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials. |
| doi_str_mv | 10.1080/15440478.2021.2009404 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_567c96a0ae3c4cf3a657e96cf434b3ee</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_567c96a0ae3c4cf3a657e96cf434b3ee</doaj_id><sourcerecordid>2738305750</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-4e8122daf18525cdcedb698f06528e0f468a32530c00960c11c0d6ec4a60b7173</originalsourceid><addsrcrecordid>eNp9UU1vFDEMHSGQKIWfgBSJ8xbnc2ZuVKUtlYqoKEjcomzibLPMJEuSEdp_T5YtPXLxsy2_Z1uv695SOKMwwHsqhQDRD2cMGG0BxlY-604O_RUI9eP5U94PL7tXpWwB2CgpO-m2l96jrSR58hE3yzyHuCGfsT4kV0iK5L7mxdYlIzHRkbucdphrwHIgfMUNRsymoiPnsT60FA25wxz3gdyH6Se5CuucQmw4zeV198KbqeCbRzztvl9dfrv4tLr9cn1zcX67slyyuhI4UMac8XSQTFpn0a3VOHhQkg0IXqjBcCY52PaoAkupBafQCqNg3dOen3Y3R12XzFbvcphN3utkgv7bSHmjTfvBTqil6u2oDBjkVljPjZI9jsp6wcWaIzatd0etXU6_FixVb9OSYztfs54PHGQvoU3J45TNqZSM_mkrBX2wSP-zSB8s0o8WNd6HIy9En_Jsfqc8OV3NfkrZZxNtKJr_X-IPBbOXhA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2738305750</pqid></control><display><type>article</type><title>Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films</title><source>Taylor and Francis Science and Technology Collection</source><creator>Yang, Hui ; Guo, Yuhang ; Zhang, Qiang ; Yan, Shuqin ; You, Renchuan</creator><creatorcontrib>Yang, Hui ; Guo, Yuhang ; Zhang, Qiang ; Yan, Shuqin ; You, Renchuan</creatorcontrib><description>The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na
2
CO
3
and NaHCO
3
are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO
3
degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na
2
CO
3
degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na
2
CO
3
degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials.</description><identifier>ISSN: 1544-0478</identifier><identifier>EISSN: 1544-046X</identifier><identifier>DOI: 10.1080/15440478.2021.2009404</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Antheraea pernyi ; Antheraea pernyi silk fibroin ; Biomaterials ; Biomedical materials ; Degradation ; degradation behavior ; Degumming ; Gel electrophoresis ; Low molecular weights ; Mechanical properties ; Molecular weight ; Proteins ; Silk ; silk biomaterials ; Silk fibroin ; Sodium bicarbonate ; Sodium carbonate ; Sodium lauryl sulfate ; 分子量 ; 力学性能 ; 柞蚕丝素蛋白 ; 脱胶 ; 蚕丝生物材料 ; 降解行为</subject><ispartof>Journal of natural fibers, 2022-11, Vol.19 (15), p.11114-11123</ispartof><rights>2021 Taylor & Francis 2021</rights><rights>2021 Taylor & Francis</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c352t-4e8122daf18525cdcedb698f06528e0f468a32530c00960c11c0d6ec4a60b7173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Guo, Yuhang</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Yan, Shuqin</creatorcontrib><creatorcontrib>You, Renchuan</creatorcontrib><title>Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films</title><title>Journal of natural fibers</title><description>The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na
2
CO
3
and NaHCO
3
are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO
3
degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na
2
CO
3
degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na
2
CO
3
degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials.</description><subject>Antheraea pernyi</subject><subject>Antheraea pernyi silk fibroin</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Degradation</subject><subject>degradation behavior</subject><subject>Degumming</subject><subject>Gel electrophoresis</subject><subject>Low molecular weights</subject><subject>Mechanical properties</subject><subject>Molecular weight</subject><subject>Proteins</subject><subject>Silk</subject><subject>silk biomaterials</subject><subject>Silk fibroin</subject><subject>Sodium bicarbonate</subject><subject>Sodium carbonate</subject><subject>Sodium lauryl sulfate</subject><subject>分子量</subject><subject>力学性能</subject><subject>柞蚕丝素蛋白</subject><subject>脱胶</subject><subject>蚕丝生物材料</subject><subject>降解行为</subject><issn>1544-0478</issn><issn>1544-046X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9UU1vFDEMHSGQKIWfgBSJ8xbnc2ZuVKUtlYqoKEjcomzibLPMJEuSEdp_T5YtPXLxsy2_Z1uv695SOKMwwHsqhQDRD2cMGG0BxlY-604O_RUI9eP5U94PL7tXpWwB2CgpO-m2l96jrSR58hE3yzyHuCGfsT4kV0iK5L7mxdYlIzHRkbucdphrwHIgfMUNRsymoiPnsT60FA25wxz3gdyH6Se5CuucQmw4zeV198KbqeCbRzztvl9dfrv4tLr9cn1zcX67slyyuhI4UMac8XSQTFpn0a3VOHhQkg0IXqjBcCY52PaoAkupBafQCqNg3dOen3Y3R12XzFbvcphN3utkgv7bSHmjTfvBTqil6u2oDBjkVljPjZI9jsp6wcWaIzatd0etXU6_FixVb9OSYztfs54PHGQvoU3J45TNqZSM_mkrBX2wSP-zSB8s0o8WNd6HIy9En_Jsfqc8OV3NfkrZZxNtKJr_X-IPBbOXhA</recordid><startdate>20221123</startdate><enddate>20221123</enddate><creator>Yang, Hui</creator><creator>Guo, Yuhang</creator><creator>Zhang, Qiang</creator><creator>Yan, Shuqin</creator><creator>You, Renchuan</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>DOA</scope></search><sort><creationdate>20221123</creationdate><title>Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films</title><author>Yang, Hui ; Guo, Yuhang ; Zhang, Qiang ; Yan, Shuqin ; You, Renchuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-4e8122daf18525cdcedb698f06528e0f468a32530c00960c11c0d6ec4a60b7173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antheraea pernyi</topic><topic>Antheraea pernyi silk fibroin</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Degradation</topic><topic>degradation behavior</topic><topic>Degumming</topic><topic>Gel electrophoresis</topic><topic>Low molecular weights</topic><topic>Mechanical properties</topic><topic>Molecular weight</topic><topic>Proteins</topic><topic>Silk</topic><topic>silk biomaterials</topic><topic>Silk fibroin</topic><topic>Sodium bicarbonate</topic><topic>Sodium carbonate</topic><topic>Sodium lauryl sulfate</topic><topic>分子量</topic><topic>力学性能</topic><topic>柞蚕丝素蛋白</topic><topic>脱胶</topic><topic>蚕丝生物材料</topic><topic>降解行为</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Guo, Yuhang</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Yan, Shuqin</creatorcontrib><creatorcontrib>You, Renchuan</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of natural fibers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Hui</au><au>Guo, Yuhang</au><au>Zhang, Qiang</au><au>Yan, Shuqin</au><au>You, Renchuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films</atitle><jtitle>Journal of natural fibers</jtitle><date>2022-11-23</date><risdate>2022</risdate><volume>19</volume><issue>15</issue><spage>11114</spage><epage>11123</epage><pages>11114-11123</pages><issn>1544-0478</issn><eissn>1544-046X</eissn><abstract>The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na
2
CO
3
and NaHCO
3
are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO
3
degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na
2
CO
3
degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na
2
CO
3
degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/15440478.2021.2009404</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor and Francis Science and Technology Collection |
| subjects | Antheraea pernyi Antheraea pernyi silk fibroin Biomaterials Biomedical materials Degradation degradation behavior Degumming Gel electrophoresis Low molecular weights Mechanical properties Molecular weight Proteins Silk silk biomaterials Silk fibroin Sodium bicarbonate Sodium carbonate Sodium lauryl sulfate 分子量 力学性能 柞蚕丝素蛋白 脱胶 蚕丝生物材料 降解行为 |
| title | Effect of Degumming Methods on Structure and Properties of Regenerated Antheraea Pernyi Silk Fibroin Films |
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