Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of Satureja macrostema (Moc. and Sessé ex Benth.) Briq

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-06, Vol.28 (12), p.4719
• Main Authors: Barrientos Ramírez, Lucia, Silva Guzmán, José Antonio, Osorio Muñoz, Edison Antonio, Alvarez Moya, Carlos, Reynoso Silva, Mónica, Cetina Corona, Abraham Francisco, Casas Solis, Josefina, Vargas Radillo, J Jesús
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial materials; Antimicrobial agents; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Assaying; Chemical composition; chemotypes; Chromatography; direct bioautography; E coli; Escherichia coli; Essential oils; Flowers & plants; Free radicals; Gas chromatography; Herbal medicine; Hydrocarbons; Lamiaceae; Lamiaceae - chemistry; Mass spectrometry; Mass spectroscopy; Monoterpenes; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; Piperitenone; Piperitenone oxide; Piperitone; piperitone derivatives; Plant Oils - chemistry; Satureja - chemistry; Satureja macrostema; Scavenging; Staphylococcus aureus; TEAC; Thin layer chromatography; Vitamin E
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28124719

is a plant that is located in various regions of Mexico and is used in a traditional way against illness. Essential oils (EOs) were obtained from leaves and the chemical composition was evaluated by gas chromatography-mass spectrometry (GC-MS). The antioxidant effect of the oil was assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by Trolox Equivalent Antioxidant Capacity (TEAC). In vitro antibacterial activity against and was determined using a broth microdilution assay and thin layer chromatography-direct bioautography (TLC-DB) to identify active antibacterial compounds. The EOs analysis showed 21 compounds, 99% terpenes, and 96% oxygenated monoterpenes, with -piperitone epoxide (46%), -piperitone epoxide (22%), and piperitenone oxide (11%) as more abundant compounds. Likewise, EOs showed an antioxidant activity of DPPH = 82%, with 50% free radical scavenging (IC ) = 7 mg/mL and TEAC = 0.005, an antibacterial effect against of 73% inhibition, and 81% over at dose of 100 µL of undiluted crude oil. The TLC-DB assay showed that the most active compounds were derived from piperitone. The comparison with other studies on shows variability in the compounds and their abundances, which can be attributed to climatic factors and the maturity of plants with similar antioxidant and antibacterial activities.