Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

• Published in: Nutrients 2016-10, Vol.8 (10), p.640-640
• Main Authors: Choi, Ji-Young, Kim, Ye Jin, Ryu, Ri, Cho, Su-Jung, Kwon, Eun-Young, Choi, Myung-Sook
• Format: Article
• Language: English
• Subjects: Adipose Tissue, White - metabolism; Adiposity; Amino acids; Amino Acids, Branched-Chain - metabolism; AMP-Activated Protein Kinases - metabolism; Animals; Body fat; Caffeine; Camellia sinensis; Diet, High-Fat - adverse effects; Dietary Supplements; Drug dosages; Dyslipidemias - drug therapy; Dyslipidemias - etiology; Dyslipidemias - genetics; Dyslipidemias - metabolism; Energy; energy expenditure; Energy Metabolism - drug effects; Fatty Liver - drug therapy; Fatty Liver - etiology; Fatty Liver - genetics; Fatty Liver - metabolism; Food; Glucose; green tea extract; Homeostasis; Homeostasis - drug effects; Insulin Resistance; Kinases; Lipids; Liver - drug effects; Male; Metabolic disorders; Mice, Inbred C57BL; Mice, Obese; Nutrition research; Obesity; Obesity - complications; Obesity - drug therapy; Obesity - genetics; Obesity - metabolism; Phytotherapy; Plant Extracts - pharmacology; Proteins; RNA; RNA-seq; Tea; Transcription, Genetic; transcriptome; Transcriptome - drug effects; Weight Gain - drug effects
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu8100640

Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% ( / ) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.