Differential expression of acetylcholinesterase 1 in response to various stress factors in honey bee workers

• Published in: Scientific reports 2019-07, Vol.9 (1), p.10342-10, Article 10342
• Main Authors: Kim, Sanghyeon, Kim, Kyungmun, Lee, Jae Ho, Han, Seung Hee, Lee, Si Hyeock
• Format: Article
• Language: English
• Subjects: 38; 38/1; 38/90; 631/601/1466; 631/80/86/2366; 82/29; 82/80; Acetylcholine receptors (muscarinic); Acetylcholinesterase; Acetylcholinesterase - genetics; Acetylcholinesterase - metabolism; Animals; Apis mellifera; Bees; Bees - genetics; Bees - physiology; Brood rearing; Cellular stress response; Genes, Insect; Heat shock proteins; Heat-Shock Response - genetics; Honey; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Hsp70 protein; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Hsp90 protein; Humanities and Social Sciences; Insect Proteins - genetics; Insect Proteins - metabolism; multidisciplinary; Physiology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Science; Science (multidisciplinary); Stress response; Stress, Physiological - genetics; Transcription factors; Transcriptome; Vitellogenin; Vitellogenins - genetics; Vitellogenins - metabolism; Workers (insect caste)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-46842-0

The honey bee acetylcholinesterase 1 (AmAChE1) has been suggested to be related to stress response as judged from its elevated expression level under brood rearing-suppressed conditions. To further investigate the involvement of AmAChE1 expression in the stress response and its physiological functions, we analyzed altered expression profiles of AmAChE1 induced by diverse stress factors. In addition, transcription profiles of several heat shock protein (Hsp) genes ( hsps ) and the vitellogenin (Vg) gene ( vg ) known as general stress markers were investigated as positive references. Among the tested stress conditions, AmAChE1 expression was induced under the brood rearing-suppressed, crowding and heat shock conditions. The hsps , particularly hsp70 and hsp90 , responded to seven of nine stress conditions tested, confirming that hsp expression profiles can serve as a general stress marker. Taken together, AmAChE1 expression is not suitable for using as a stress marker due to its limited response. Nevertheless, AmAChE1 expression appears to be connected, at least in part, to heat shock response and other pathways. Considering that AmAChE1 likely regulates the ACh titer particularly in non-neuronal tissues, thereby modulating the signal cascades mediated by mAChR, the AmAChE1 expression profile under different conditions likely provides important information on its physiological roles in honey bees.