Transcriptional Inhibition of lncRNA gadd7 by CRISPR/dCas9-KRAB Protects Spermatocyte Viability

Our previous study found that lncRNA gadd7 was up-regulated in the semen of varicocele patients, and could promote the apoptosis of mouse spermatocytes and inhibit their proliferation. Therefore, we further explored whether down-regulation of Gadd seven expression could protect the viability of sper...

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Bibliographic Details
Published in:Frontiers in molecular biosciences 2021-03, Vol.8, p.652392-652392
Main Authors: Zhao, Jun, Ma, Wenmin, Zhong, Yucheng, Deng, Hao, Zhou, Bingyu, Wu, Yaqin, Yang, Meiqiong, Li, Huan
Format: Article
Language:English
Subjects:
CRISPR
infertility
lncRNA gadd7
male
Molecular Biosciences
varicocele
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Summary:Our previous study found that lncRNA gadd7 was up-regulated in the semen of varicocele patients, and could promote the apoptosis of mouse spermatocytes and inhibit their proliferation. Therefore, we further explored whether down-regulation of Gadd seven expression could protect the viability of spermatocytes. Here we designed specific sgRNAs targeting the ORF region of gadd7, and constructed a CRISPR-dCas9-KRAB system that effectively inhibits gadd7 at the transcriptional level. The CRISPRi system can effectively prevent the apoptosis of spermatocytes and enhance their proliferation, which is expected to provide a potentially effective molecular intervention method for the treatment of male infertility caused by varicocele.
ISSN:2296-889X
2296-889X
DOI:10.3389/fmolb.2021.652392