The Bacteriophage Pf-10-A Component of the Biopesticide "Multiphage" Used to Control Agricultural Crop Diseases Caused by Pseudomonas syringae

• Published in: Viruses 2021-12, Vol.14 (1), p.42
• Main Authors: Kazantseva, Olesya A, Buzikov, Rustam M, Pilipchuk, Tatsiana A, Valentovich, Leonid N, Kazantsev, Andrey N, Kalamiyets, Emilia I, Shadrin, Andrey M
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial infections; bacteriophage; Bacteriophages - classification; Bacteriophages - genetics; Bacteriophages - isolation & purification; Bacteriophages - physiology; Base Composition; Biological Control Agents; biopesticide; Caudovirales - genetics; Crop diseases; Crops; Crops, Agricultural - microbiology; DNA, Viral - genetics; Genome, Viral; Host Specificity; Microorganisms; Pathogens; phage; Phages; Phylogenetics; Phylogeny; phytopathogenic bacteria; Plant diseases; Plant Diseases - microbiology; Plant Diseases - prevention & control; Podoviridae - genetics; Polyethylene glycol; Pseudomonas; Pseudomonas Phages - classification; Pseudomonas Phages - genetics; Pseudomonas Phages - isolation & purification; Pseudomonas Phages - physiology; Pseudomonas syringae; Pseudomonas syringae - virology; Siphoviridae - genetics; Virion; Virions
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v14010042

Phytopathogenic pseudomonads are widespread in the world and cause a wide range of plant diseases. In this work, we describe the phage Pf-10, which is a part of the biopesticide "Multiphage" used for bacterial diseases of agricultural crops caused by . The Pf-10 chromosome is a dsDNA molecule with two direct terminal repeats (DTRs). The phage genomic DNA is 39,424 bp long with a GC-content of 56.5%. The Pf-10 phage uses a packaging mechanism based on T7-like short DTRs, and the length of each terminal repeat is 257 bp. Electron microscopic analysis has shown that phage Pf-10 has the podovirus morphotype. Phage Pf-10 is highly stable at pH values from 5 to 10 and temperatures from 4 to 60 °C and has a lytic activity against strains. Phage Pf-10 is characterized by fast adsorption rate (80% of virions attach to the host cells in 10 min), but has a relatively small number of progeny (37 ± 8.5 phage particles per infected cell). According to the phylogenetic analysis, phage Pf-10 can be classified as a new phage species belonging to the genus , subfamily , family , order .