The effect of postmenopausal osteoporosis on subchondral bone pathology in a rat model of knee osteoarthritis

This study aimed to investigate the additional effect of ovariectomy-induced osteoporosis (OP) on the pathology of knee osteoarthritis (OA) in a rat meniscectomized model, particularly focusing on subchondral bone changes and pain behaviour. Rats were divided into four groups, sham, OP, OA, OP plus...

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Bibliographic Details
Published in:Scientific reports 2023-02, Vol.13 (1), p.2926-2926, Article 2926
Main Authors: Wada, Hiroyuki, Aso, Koji, Izumi, Masashi, Ikeuchi, Masahiko
Format: Article
Language:English
Subjects:
692/308/1426
692/699/1670/316
692/699/1670/407
Animals
Arthritis
Bone and Bones - metabolism
Calcification
Cancellous bone
Cartilage
Cartilage - metabolism
Cartilage diseases
Cartilage, Articular - pathology
Female
Histology
Humanities and Social Sciences
Humans
Knee
multidisciplinary
NF-κB protein
Osteoarthritis
Osteoarthritis, Knee - pathology
Osteoclasts
Osteoporosis
Osteoporosis - metabolism
Osteoporosis, Postmenopausal - metabolism
Osteoprotegerin
Ovariectomy
Pain
Pain perception
Pathology
Post-menopause
Rats
Science
Science (multidisciplinary)
Subchondral bone
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Summary:This study aimed to investigate the additional effect of ovariectomy-induced osteoporosis (OP) on the pathology of knee osteoarthritis (OA) in a rat meniscectomized model, particularly focusing on subchondral bone changes and pain behaviour. Rats were divided into four groups, sham, OP, OA, OP plus OA, and assessed for histology, osteoclast activity, subchondral bone microstructure, and pain-related behaviour. Rats with OP plus OA had significantly increased calcified cartilage and subchondral bone damage scores, increased densities of subchondral osteoclasts in the weight-bearing area, and more porous subchondral trabecular bone compared with rats with OA. Loss of tidemark integrity was observed most frequently in rats with OP plus OA. The density of subchondral osteoclasts correlated with the calcified cartilage and subchondral bone damage score in rats with OA (OA and OP plus OA). No significant differences in the receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) expression ratio in subchondral bone and pain-related behavioural tests were observed between rats with OA and rats with OP plus OA. In rats with OA, coexisting OP potentially aggravated OA pathology mainly in calcified cartilage and subchondral trabecular bone by increasing subchondral osteoclast activity.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-29802-7