Metabolic modulation to improve MSC expansion and therapeutic potential for articular cartilage repair

• Published in: Stem cell research & therapy 2024-09, Vol.15 (1), p.308-13, Article 308
• Main Authors: Tee, Ching Ann, Roxby, Daniel Ninio, Othman, Rashidah, Denslin, Vinitha, Bhat, Kiesar Sideeq, Yang, Zheng, Han, Jongyoon, Tucker-Kellogg, Lisa, Boyer, Laurie A
• Format: Article
• Language: English
• Subjects: Animals; Arthritis; Articular cartilage; Cartilage, Articular - metabolism; Cell Differentiation; Cell expansion; Cell Proliferation; Cells, Cultured; Chondrocytes - cytology; Chondrocytes - metabolism; Chondrogenesis - drug effects; Critical quality attributes; Dextrose; Glucose; Glutamine; Health aspects; Humans; In-process monitoring tools; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchymal stromal cells; Metabolic modulation; Phosphates; Stem cells
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-024-03923-w

Articular cartilage degeneration can result from injury, age, or arthritis, causing significant joint pain and disability without surgical intervention. Currently, the only FDA cell-based therapy for articular cartilage injury is Autologous Chondrocyte Implantation (ACI); however, this procedure is costly, time-intensive, and requires multiple treatments. Mesenchymal stromal cells (MSCs) are an attractive alternative autologous therapy due to their availability and ability to robustly differentiate into chondrocytes for transplantation with good safety profiles. However, treatment outcomes are variable due to donor-to-donor variability as well as intrapopulation heterogeneity and unstandardized MSC manufacturing protocols. Process improvements that reduce cell heterogeneity while increasing donor cell numbers with improved chondrogenic potential during expansion culture are needed to realize the full potential of MSC therapy. In this study, we investigated the potential of MSC metabolic modulation during expansion to enhance their chondrogenic commitment by varying the nutrient composition, including glucose, pyruvate, glutamine, and ascorbic acid in culture media. We tested the effect of metabolic modulation in short-term (one passage) and long-term (up to seven passages). We measured metabolic state, cell size, population doubling time, and senescence and employed novel tools including micro-magnetic resonance relaxometry (µMRR) relaxation time (T ) to characterize the effects of AA on improved MSC expansion and chondrogenic potential. Our data show that the addition of 1 mM L-ascorbic acid-2-phosphate (AA) to cultures for one passage during MSC expansion prior to initiation of differentiation improves chondrogenic differentiation. We further demonstrate that AA treatment reduced the proportion of senescent cells and cell heterogeneity also allowing for long-term expansion that led to a > 300-fold increase in yield of MSCs with enhanced chondrogenic potential compared to untreated cells. AA-treated MSCs with improved chondrogenic potential showed a robust shift in metabolic profile to OXPHOS and higher µMRR T values, identifying critical quality attributes that could be implemented in MSC manufacturing for articular cartilage repair. Our results suggest an improved MSC manufacturing process that can enhance chondrogenic potential by targeting MSC metabolism and integrating process analytic tools during expansion.