A non-targeted metabolomics analysis identifies wound-induced oxylipins in Physcomitrium patens

• Published in: Frontiers in plant science 2023-01, Vol.13, p.1085915
• Main Authors: Resemann, Hanno Christoph, Feussner, Kirstin, Hornung, Ellen, Feussner, Ivo
• Format: Article
• Language: English
• Subjects: bryophyte; cyclopentenones; lipid peroxidation; metabolite fingerprinting; octadecanoids; Physcomitrella patens; Plant Science
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.1085915

Plant oxylipins are a class of lipid-derived signaling molecules being involved in the regulation of various biotic and abiotic stress responses. A major class of oxylipins are the circular derivatives to which 12-oxo-phytodienoic acid (OPDA) and its metabolite jasmonic acid (JA) belong. While OPDA and its shorter chain homologue -OPDA (dnOPDA) seem to be ubiquitously found in land plants ranging from bryophytes to angiosperms, the occurrence of JA and its derivatives is still under discussion. The bryophyte has received increased scientific interest as a non-vascular plant model organism over the last decade. Therefore, we followed the metabolism upon wounding by metabolite fingerprinting with the aim to identify jasmonates as well as novel oxylipins in . A non-targeted metabolomics approach was used to reconstruct the metabolic pathways for the synthesis of oxylipins, derived from roughanic, linoleic, α-linolenic, and arachidonic acid in wild type, the oxylipin-deficient mutants of and , the mutants of being deficient in all fatty acids harboring a Δ -double bond and the C20-fatty acid-deficient mutants of . Beside of OPDA, -OPDA, dnOPDA, and -dnOPDA, three additional C18-compounds and a metabolite being isobaric to JA were identified to accumulate after wounding. These findings can now serve as foundation for future research in determining, which compound(s) will serve as native ligand(s) for the oxylipin-receptor COI1 in .