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Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities
Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from , harvested from the flowers of mimosa ( L.) in the Chiang Mai pro...
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| Published in: | Journal of Apicultural Science 2021-06, Vol.65 (1), p.71-83 |
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| Main Authors: | , , , |
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| cites | cdi_FETCH-LOGICAL-c421t-cc945f167c5fee02c2636ac29a98efea635ca9eb7070272a2b88f43c039acc543 |
| container_end_page | 83 |
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| container_start_page | 71 |
| container_title | Journal of Apicultural Science |
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| creator | Khongkarat, Phanthiwa Ramadhan, Rico Phuwapraisirisan, Preecha Chanchao, Chanpen |
| description | Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from
, harvested from the flowers of mimosa (
L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC
) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC
of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC
of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC
of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%). |
| doi_str_mv | 10.2478/jas-2021-0001 |
| format | article |
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, harvested from the flowers of mimosa (
L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC
) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC
of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC
of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC
of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).</description><identifier>ISSN: 2299-4831</identifier><identifier>ISSN: 1643-4439</identifier><identifier>EISSN: 2299-4831</identifier><identifier>DOI: 10.2478/jas-2021-0001</identifier><language>eng</language><publisher>Pulawy: Sciendo</publisher><subject>Antioxidants ; bee pollen ; Bees ; Biogeography ; Chromatography ; Column chromatography ; Dichloromethane ; Flavonoids ; Flowers ; Fractionation ; Hexanes ; Kojic acid ; mimosa ; Mimosa pigra ; Mushrooms ; Naringenin ; NMR ; Nuclear magnetic resonance ; partitioned extract ; Pollen ; Silica ; Silica gel ; Silicon dioxide ; Size exclusion chromatography ; Tyrosinase</subject><ispartof>Journal of Apicultural Science, 2021-06, Vol.65 (1), p.71-83</ispartof><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-cc945f167c5fee02c2636ac29a98efea635ca9eb7070272a2b88f43c039acc543</citedby><cites>FETCH-LOGICAL-c421t-cc945f167c5fee02c2636ac29a98efea635ca9eb7070272a2b88f43c039acc543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2544566660?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,25731,27901,27902,36989,44566</link.rule.ids></links><search><creatorcontrib>Khongkarat, Phanthiwa</creatorcontrib><creatorcontrib>Ramadhan, Rico</creatorcontrib><creatorcontrib>Phuwapraisirisan, Preecha</creatorcontrib><creatorcontrib>Chanchao, Chanpen</creatorcontrib><title>Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities</title><title>Journal of Apicultural Science</title><description>Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from
, harvested from the flowers of mimosa (
L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC
) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC
of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC
of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC
of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).</description><subject>Antioxidants</subject><subject>bee pollen</subject><subject>Bees</subject><subject>Biogeography</subject><subject>Chromatography</subject><subject>Column chromatography</subject><subject>Dichloromethane</subject><subject>Flavonoids</subject><subject>Flowers</subject><subject>Fractionation</subject><subject>Hexanes</subject><subject>Kojic acid</subject><subject>mimosa</subject><subject>Mimosa pigra</subject><subject>Mushrooms</subject><subject>Naringenin</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>partitioned extract</subject><subject>Pollen</subject><subject>Silica</subject><subject>Silica gel</subject><subject>Silicon dioxide</subject><subject>Size exclusion chromatography</subject><subject>Tyrosinase</subject><issn>2299-4831</issn><issn>1643-4439</issn><issn>2299-4831</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkU1P3DAQhqOqlUCUI3dLnEP9nbi3BUGLtFWRoGdr1pmkXgV7sbMs--_rsKj0UB_ssfX4GVtvVZ0xesFl035ZQ6455aymlLIP1THnxtSyFezjP_VRdZrzuhBUt9IIdVzle5cQgw8DgdCRSx-Hre-wIzcJ3ORjgHkisSc__GPMQDZ-SECWF-QSkdzFccRAdn76TRahkC--gzC9quZ9_bBPMfsAGcmi6J795DF_rj71MGY8fVtPql831w9X3-vlz2-3V4tl7SRnU-2ckapnunGqR6TccS00OG7AtNgjaKEcGFw1tKG84cBXbdtL4agw4JyS4qS6PXi7CGu7Sf4R0t5G8Pb1IKbBQpq8G9GqhjnDUAlkRpqWGla6da2Wwqw6zVRxnR9cmxSftpgnu47bFMrzLVdSKl0GLVR9oFz5dk7Y_-3KqJ1jsiUmO8dk55gK__XA72CcMHU4pO2-FO_y_97TijVM_AEvt5fo</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Khongkarat, Phanthiwa</creator><creator>Ramadhan, Rico</creator><creator>Phuwapraisirisan, Preecha</creator><creator>Chanchao, Chanpen</creator><general>Sciendo</general><general>De Gruyter Poland</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20210601</creationdate><title>Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities</title><author>Khongkarat, Phanthiwa ; Ramadhan, Rico ; Phuwapraisirisan, Preecha ; Chanchao, Chanpen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-cc945f167c5fee02c2636ac29a98efea635ca9eb7070272a2b88f43c039acc543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antioxidants</topic><topic>bee pollen</topic><topic>Bees</topic><topic>Biogeography</topic><topic>Chromatography</topic><topic>Column chromatography</topic><topic>Dichloromethane</topic><topic>Flavonoids</topic><topic>Flowers</topic><topic>Fractionation</topic><topic>Hexanes</topic><topic>Kojic acid</topic><topic>mimosa</topic><topic>Mimosa pigra</topic><topic>Mushrooms</topic><topic>Naringenin</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>partitioned extract</topic><topic>Pollen</topic><topic>Silica</topic><topic>Silica gel</topic><topic>Silicon dioxide</topic><topic>Size exclusion chromatography</topic><topic>Tyrosinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khongkarat, Phanthiwa</creatorcontrib><creatorcontrib>Ramadhan, Rico</creatorcontrib><creatorcontrib>Phuwapraisirisan, Preecha</creatorcontrib><creatorcontrib>Chanchao, Chanpen</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Agriculture Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of Apicultural Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khongkarat, Phanthiwa</au><au>Ramadhan, Rico</au><au>Phuwapraisirisan, Preecha</au><au>Chanchao, Chanpen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities</atitle><jtitle>Journal of Apicultural Science</jtitle><date>2021-06-01</date><risdate>2021</risdate><volume>65</volume><issue>1</issue><spage>71</spage><epage>83</epage><pages>71-83</pages><issn>2299-4831</issn><issn>1643-4439</issn><eissn>2299-4831</eissn><abstract>Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from
, harvested from the flowers of mimosa (
L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC
) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC
of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC
of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC
of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).</abstract><cop>Pulawy</cop><pub>Sciendo</pub><doi>10.2478/jas-2021-0001</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2299-4831 |
| ispartof | Journal of Apicultural Science, 2021-06, Vol.65 (1), p.71-83 |
| issn | 2299-4831 1643-4439 2299-4831 |
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| source | Publicly Available Content Database; EZB Electronic Journals Library |
| subjects | Antioxidants bee pollen Bees Biogeography Chromatography Column chromatography Dichloromethane Flavonoids Flowers Fractionation Hexanes Kojic acid mimosa Mimosa pigra Mushrooms Naringenin NMR Nuclear magnetic resonance partitioned extract Pollen Silica Silica gel Silicon dioxide Size exclusion chromatography Tyrosinase |
| title | Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities |
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