Neural regulation in tooth regeneration of Ambystoma mexicanum

• Published in: Scientific reports 2020-06, Vol.10 (1), p.9323-9323, Article 9323
• Main Authors: Makanae, Aki, Tajika, Yuki, Nishimura, Koki, Saito, Nanami, Tanaka, Jun-ichi, Satoh, Akira
• Format: Article
• Language: English
• Subjects: 631/136/142; 631/136/1660; 631/136/2091; Ambystoma mexicanum; Ambystoma mexicanum - genetics; Ambystoma mexicanum - physiology; Amphibians; Animals; Animals, Genetically Modified; Bone Morphogenetic Protein 2 - genetics; Bone morphogenetic protein 7; Bone Morphogenetic Protein 7 - genetics; Bones; Cartilage; Cell growth; Denervation; Enamel; Fibroblast growth factor 2; Fibroblast Growth Factor 2 - genetics; Fibroblast growth factor 8; Fibroblast Growth Factor 8 - genetics; Gills; Green Fluorescent Proteins - genetics; Hedgehog Proteins - genetics; Humanities and Social Sciences; Imaging, Three-Dimensional; Jaw; Limbs; Mandible; Mandible - innervation; Mandible - surgery; Morphology; multidisciplinary; Nerves; Odontoblasts - cytology; Regeneration; Regeneration - physiology; Science; Science (multidisciplinary); Teeth; Tooth - anatomy & histology; Tooth - physiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-66142-2

The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.