GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana

Drought transcriptome analysis of finger millet ( Eleusine coracana ) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize ( Zea mays ), several genes, including a G-BOX B...

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Bibliographic Details
Published in:Scientific reports 2017-08, Vol.7 (1), p.9148-9148, Article 9148
Main Authors: Ramegowda, Venkategowda, Gill, Upinder Singh, Sivalingam, Palaiyur Nanjappan, Gupta, Aarti, Gupta, Chirag, Govind, Geetha, Nataraja, Karaba N., Pereira, Andy, Udayakumar, Makarla, Mysore, Kirankumar S., Senthil-Kumar, Muthappa
Format: Article
Language:English
Subjects:
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631/1647/1511
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Arabidopsis thaliana
Cereal crops
Drought
Drought resistance
Eleusine coracana
Gene silencing
Genes
Humanities and Social Sciences
Millet
multidisciplinary
Osmotic stress
Plant species
Science
Science (multidisciplinary)
Transgenic plants
Zea mays
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Summary:Drought transcriptome analysis of finger millet ( Eleusine coracana ) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize ( Zea mays ), several genes, including a G-BOX BINDING FACTOR 3 ( GBF3 ) were identified as candidate drought stress response genes and the role of GBF3 in drought tolerance was studied in Arabidopsis thaliana . Overexpression of both EcGBF3 and AtGBF3 in A. thaliana resulted in improved tolerance to osmotic stress, salinity and drought stress in addition to conferring insensitivity to ABA. Conversely, loss of function of this gene increased the sensitivity of A. thaliana plants to drought stress. EcGBF3 transgenic A. thaliana results also suggest that drought tolerance of sensitive plants can be improved by transferring genes from far related crops like finger millet. Our results demonstrate the role of GBF3 in imparting drought tolerance in A. thaliana and indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-09542-1