Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis

The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers. The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell typ...

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Published in:TheScientificWorld 2011-01, Vol.2011 (2011), p.1-10
Main Authors: Megat Abdul Wahab, Rohaya, Zainal Ariffin, Zaidah, Senafi, Sahidan, Zainal Ariffin, Shahrul Hisham, Yazid, Muhammad Dain
Format: Article
Language:English
Subjects:
Animals
Antigens, Ly - genetics
Blood
Culture Media
Endoglin
Health aspects
Intracellular Signaling Peptides and Proteins - genetics
Membrane Proteins - genetics
Mice
Molecular dynamics
Physiological aspects
Polymerase Chain Reaction
Stem cells
Stem Cells - cytology
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cited_by cdi_FETCH-LOGICAL-c637t-6e0a765fd28eb1207e76bdce0aef4004cdb645c56ab44bfc8096d883f3eabbb03
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container_title TheScientificWorld
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creator Megat Abdul Wahab, Rohaya
Zainal Ariffin, Zaidah
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Yazid, Muhammad Dain
description The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers. The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell types, that is, adherent and suspension. Following a total culture time of 14 days, adherent cells activated the Cd105 gene while suspension cells activated the Sca-1 gene. Both progenitor markers, Cbfa-1 and Ostf-1, were inactivated in both suspension and adherent cells after 14-day culture compared to cells cultured 3 days in designated differentiation medium. In conclusion, molecular analyses showed that primary mononucleated cells are heterogeneous, consisting of hematopoietic stem cells (suspension) and mesenchymal stem cells (adherent) while both cells contained no progenitor cells.
doi_str_mv 10.1100/2011/340278
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subjects Animals
Antigens, Ly - genetics
Blood
Culture Media
Endoglin
Health aspects
Intracellular Signaling Peptides and Proteins - genetics
Membrane Proteins - genetics
Mice
Molecular dynamics
Physiological aspects
Polymerase Chain Reaction
Stem cells
Stem Cells - cytology
title Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis
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