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Veratryl Alcohol Attenuates the Virulence and Pathogenicity of Pseudomonas aeruginosa Mainly via Targeting las Quorum-Sensing System
is an opportunistic pathogen that usually causes chronic infections and even death in patients. The treatment of infection has become more challenging due to the prevalence of antibiotic resistance and the slow pace of new antibiotic development. Therefore, it is essential to explore non-antibiotic...
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| Published in: | Microorganisms (Basel) 2024-05, Vol.12 (5), p.985 |
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| container_issue | 5 |
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| container_title | Microorganisms (Basel) |
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| creator | Fu, Songzhe Song, Wenxu Han, Xiaofeng Chen, Lin Shen, Lixin |
| description | is an opportunistic pathogen that usually causes chronic infections and even death in patients. The treatment of
infection has become more challenging due to the prevalence of antibiotic resistance and the slow pace of new antibiotic development. Therefore, it is essential to explore non-antibiotic methods. A new strategy involves screening for drugs that target the quorum-sensing (QS) system. The QS system regulates the infection and drug resistance in
. In this study, veratryl alcohol (VA) was found as an effective QS inhibitor (QSI). It effectively suppressed the expression of QS-related genes and the subsequent production of virulence factors under the control of QS including elastase, protease, pyocyanin and rhamnolipid at sub-inhibitory concentrations. In addition, motility activity and biofilm formation, which were correlated with the infection of
, were also suppressed by VA. In vivo experiments demonstrated that VA could weaken the pathogenicity of
in Chinese cabbage,
, and
infection models. Molecular docking, combined with QS quintuple mutant infection analysis, identified that the mechanism of VA could target the LasR protein of the
system mainly. Moreover, VA increased the susceptibility of
to conventional antibiotics of tobramycin, kanamycin and gentamicin. The results firstly demonstrate that VA is a promising QSI to treat infections caused by
. |
| doi_str_mv | 10.3390/microorganisms12050985 |
| format | article |
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infection has become more challenging due to the prevalence of antibiotic resistance and the slow pace of new antibiotic development. Therefore, it is essential to explore non-antibiotic methods. A new strategy involves screening for drugs that target the quorum-sensing (QS) system. The QS system regulates the infection and drug resistance in
. In this study, veratryl alcohol (VA) was found as an effective QS inhibitor (QSI). It effectively suppressed the expression of QS-related genes and the subsequent production of virulence factors under the control of QS including elastase, protease, pyocyanin and rhamnolipid at sub-inhibitory concentrations. In addition, motility activity and biofilm formation, which were correlated with the infection of
, were also suppressed by VA. In vivo experiments demonstrated that VA could weaken the pathogenicity of
in Chinese cabbage,
, and
infection models. Molecular docking, combined with QS quintuple mutant infection analysis, identified that the mechanism of VA could target the LasR protein of the
system mainly. Moreover, VA increased the susceptibility of
to conventional antibiotics of tobramycin, kanamycin and gentamicin. The results firstly demonstrate that VA is a promising QSI to treat infections caused by
.</description><identifier>ISSN: 2076-2607</identifier><identifier>EISSN: 2076-2607</identifier><identifier>DOI: 10.3390/microorganisms12050985</identifier><identifier>PMID: 38792814</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alcohol ; Antibiotic resistance ; Antibiotics ; Bacteria ; Bacterial infections ; Biofilms ; Chinese cabbage ; Drug resistance ; Drug screening ; Elastase ; Gene expression ; Gentamicin ; Kanamycin ; LasR protein ; Metabolites ; Molecular docking ; Molecular modelling ; Motility ; Nosocomial infections ; Opportunist infection ; Pathogenicity ; Pathogens ; Pseudomonas aeruginosa ; Pyocyanin ; quorum sensing ; Tobramycin ; Veratryl alcohol ; Virulence ; Virulence factors</subject><ispartof>Microorganisms (Basel), 2024-05, Vol.12 (5), p.985</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c330t-d717e12a9ae08aa73ae7ecc134f131dcc52c62159e63a43a7ba5e2f986ddac9a3</cites><orcidid>0000-0003-2322-4595</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3059590279/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3059590279?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38792814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Songzhe</creatorcontrib><creatorcontrib>Song, Wenxu</creatorcontrib><creatorcontrib>Han, Xiaofeng</creatorcontrib><creatorcontrib>Chen, Lin</creatorcontrib><creatorcontrib>Shen, Lixin</creatorcontrib><title>Veratryl Alcohol Attenuates the Virulence and Pathogenicity of Pseudomonas aeruginosa Mainly via Targeting las Quorum-Sensing System</title><title>Microorganisms (Basel)</title><addtitle>Microorganisms</addtitle><description>is an opportunistic pathogen that usually causes chronic infections and even death in patients. The treatment of
infection has become more challenging due to the prevalence of antibiotic resistance and the slow pace of new antibiotic development. Therefore, it is essential to explore non-antibiotic methods. A new strategy involves screening for drugs that target the quorum-sensing (QS) system. The QS system regulates the infection and drug resistance in
. In this study, veratryl alcohol (VA) was found as an effective QS inhibitor (QSI). It effectively suppressed the expression of QS-related genes and the subsequent production of virulence factors under the control of QS including elastase, protease, pyocyanin and rhamnolipid at sub-inhibitory concentrations. In addition, motility activity and biofilm formation, which were correlated with the infection of
, were also suppressed by VA. In vivo experiments demonstrated that VA could weaken the pathogenicity of
in Chinese cabbage,
, and
infection models. Molecular docking, combined with QS quintuple mutant infection analysis, identified that the mechanism of VA could target the LasR protein of the
system mainly. Moreover, VA increased the susceptibility of
to conventional antibiotics of tobramycin, kanamycin and gentamicin. The results firstly demonstrate that VA is a promising QSI to treat infections caused by
.</description><subject>Alcohol</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biofilms</subject><subject>Chinese cabbage</subject><subject>Drug resistance</subject><subject>Drug screening</subject><subject>Elastase</subject><subject>Gene expression</subject><subject>Gentamicin</subject><subject>Kanamycin</subject><subject>LasR protein</subject><subject>Metabolites</subject><subject>Molecular docking</subject><subject>Molecular modelling</subject><subject>Motility</subject><subject>Nosocomial infections</subject><subject>Opportunist infection</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pseudomonas aeruginosa</subject><subject>Pyocyanin</subject><subject>quorum sensing</subject><subject>Tobramycin</subject><subject>Veratryl alcohol</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>2076-2607</issn><issn>2076-2607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkctq3TAQhk1pSUKaVwiCbrpxq4ttScsQegmkNCGXrZkjj310sKVUl4L3ffAqPWkoJbOZYfjmZ2b-qjpl9IMQmn5crAnehwmcjUtknLZUq_ZVdcSp7GreUfn6n_qwOolxR0toJlTLDqpDoaTmijVH1a97DJDCOpOz2fitLzkldBkSRpK2SO5tyDM6gwTcQK4gbf2EzhqbVuJHchUxD37xDiIBDHmyzkcg38C6eSU_LZBbCBMm6yYyF-Y6-5CX-gZdfGzdrDHh8rZ6M8Ic8eQpH1d3nz_dnn-tL79_uTg_u6yNEDTVg2QSGQcNSBWAFIASjWGiGZlggzEtNx1nrcZOQCNAbqBFPmrVDQMYDeK4utjrDh52_UOwC4S192D7P43y0R5CsmbGvpWdoJtx7DTjjUK2UaYxyKWBQSgKomi932s9BP8jY0z9YqPBeQaHPsde0I4KSZXiBX33H7rzObhyaaFa3WrKpS5Ut6eKtzEGHJ8XZLR_tL1_2fYyePoknzcLDs9jf00WvwFv7q-O</recordid><startdate>20240514</startdate><enddate>20240514</enddate><creator>Fu, Songzhe</creator><creator>Song, Wenxu</creator><creator>Han, Xiaofeng</creator><creator>Chen, Lin</creator><creator>Shen, Lixin</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2322-4595</orcidid></search><sort><creationdate>20240514</creationdate><title>Veratryl Alcohol Attenuates the Virulence and Pathogenicity of Pseudomonas aeruginosa Mainly via Targeting las Quorum-Sensing System</title><author>Fu, Songzhe ; Song, Wenxu ; Han, Xiaofeng ; Chen, Lin ; Shen, Lixin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-d717e12a9ae08aa73ae7ecc134f131dcc52c62159e63a43a7ba5e2f986ddac9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alcohol</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biofilms</topic><topic>Chinese cabbage</topic><topic>Drug resistance</topic><topic>Drug screening</topic><topic>Elastase</topic><topic>Gene expression</topic><topic>Gentamicin</topic><topic>Kanamycin</topic><topic>LasR protein</topic><topic>Metabolites</topic><topic>Molecular docking</topic><topic>Molecular modelling</topic><topic>Motility</topic><topic>Nosocomial infections</topic><topic>Opportunist infection</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Pseudomonas aeruginosa</topic><topic>Pyocyanin</topic><topic>quorum sensing</topic><topic>Tobramycin</topic><topic>Veratryl alcohol</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Songzhe</creatorcontrib><creatorcontrib>Song, Wenxu</creatorcontrib><creatorcontrib>Han, Xiaofeng</creatorcontrib><creatorcontrib>Chen, Lin</creatorcontrib><creatorcontrib>Shen, Lixin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Microorganisms (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Songzhe</au><au>Song, Wenxu</au><au>Han, Xiaofeng</au><au>Chen, Lin</au><au>Shen, Lixin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Veratryl Alcohol Attenuates the Virulence and Pathogenicity of Pseudomonas aeruginosa Mainly via Targeting las Quorum-Sensing System</atitle><jtitle>Microorganisms (Basel)</jtitle><addtitle>Microorganisms</addtitle><date>2024-05-14</date><risdate>2024</risdate><volume>12</volume><issue>5</issue><spage>985</spage><pages>985-</pages><issn>2076-2607</issn><eissn>2076-2607</eissn><abstract>is an opportunistic pathogen that usually causes chronic infections and even death in patients. The treatment of
infection has become more challenging due to the prevalence of antibiotic resistance and the slow pace of new antibiotic development. Therefore, it is essential to explore non-antibiotic methods. A new strategy involves screening for drugs that target the quorum-sensing (QS) system. The QS system regulates the infection and drug resistance in
. In this study, veratryl alcohol (VA) was found as an effective QS inhibitor (QSI). It effectively suppressed the expression of QS-related genes and the subsequent production of virulence factors under the control of QS including elastase, protease, pyocyanin and rhamnolipid at sub-inhibitory concentrations. In addition, motility activity and biofilm formation, which were correlated with the infection of
, were also suppressed by VA. In vivo experiments demonstrated that VA could weaken the pathogenicity of
in Chinese cabbage,
, and
infection models. Molecular docking, combined with QS quintuple mutant infection analysis, identified that the mechanism of VA could target the LasR protein of the
system mainly. Moreover, VA increased the susceptibility of
to conventional antibiotics of tobramycin, kanamycin and gentamicin. The results firstly demonstrate that VA is a promising QSI to treat infections caused by
.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38792814</pmid><doi>10.3390/microorganisms12050985</doi><orcidid>https://orcid.org/0000-0003-2322-4595</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Alcohol Antibiotic resistance Antibiotics Bacteria Bacterial infections Biofilms Chinese cabbage Drug resistance Drug screening Elastase Gene expression Gentamicin Kanamycin LasR protein Metabolites Molecular docking Molecular modelling Motility Nosocomial infections Opportunist infection Pathogenicity Pathogens Pseudomonas aeruginosa Pyocyanin quorum sensing Tobramycin Veratryl alcohol Virulence Virulence factors |
| title | Veratryl Alcohol Attenuates the Virulence and Pathogenicity of Pseudomonas aeruginosa Mainly via Targeting las Quorum-Sensing System |
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